Transcription Factor WRKY75 Interacts with DELLA Proteins to Affect Flowering
Zhang, Liping1,2; Chen, Ligang2; Yu, Diqiu2
2018
发表期刊PLANT PHYSIOLOGY
ISSN0032-0889
卷号176期号:1页码:790-803
摘要Flowering time is tightly controlled by both endogenous and exogenous signals. Although several lines of evidence have suggested the involvement of WRKY transcription factors in floral initiation, the underlying mechanisms and signaling pathways involved remain elusive. Here, we newly identified Arabidopsis (Arabidopsis thaliana) WRKY DNA binding protein75 (WRKY75) as a positive regulator of flowering initiation. Mutation of WRKY75 resulted in a delay in flowering, whereas overexpression of WRKY75 significantly accelerated flowering in Arabidopsis. Gene expression analysis showed that the transcript abundance of the flowering time integrator gene FLOWERING LOCUS T (FT) was lower in wrky75 mutants than in the wild type, but greater in WRKY75-overexpressing plants. Chromatin immunoprecipitation assays revealed that WRKY75 directly binds to the promoter of FT. Both in vivo and in vitro biochemical analyses demonstrated that WRKY75 interacts with DELLA proteins. We found that both REPRESSOR OF ga1-3 (RGA) RGA-LIKE1 (RGL1) and GA INSENSITIVE (GAI) can repress the activation ability of WRKY75, thereby attenuating expression of its regulon. Genetic analyses indicated that WRKY75 positively regulates flowering in a FT-dependent manner and overexpression of RGL1 or gain-of-function of GAI could partially rescue the early flowering phenotype of WRKY75-overexpressing plants. Taken together, our results demonstrate that WRKY75 may function as a new component of the GA-mediated signaling pathway to positively regulate flowering in Arabidopsis.
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/60598
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Plant Resources & Sustainable Use, Kunming 650223, Yunnan, Peoples R China
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Zhang, Liping,Chen, Ligang,Yu, Diqiu. Transcription Factor WRKY75 Interacts with DELLA Proteins to Affect Flowering[J]. PLANT PHYSIOLOGY,2018,176(1):790-803.
APA Zhang, Liping,Chen, Ligang,&Yu, Diqiu.(2018).Transcription Factor WRKY75 Interacts with DELLA Proteins to Affect Flowering.PLANT PHYSIOLOGY,176(1),790-803.
MLA Zhang, Liping,et al."Transcription Factor WRKY75 Interacts with DELLA Proteins to Affect Flowering".PLANT PHYSIOLOGY 176.1(2018):790-803.
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