Development of 13 microsatellite markers for Castanopsis tribuloides (Fagaceae) using next-generation sequencing

doi:10.1007/s11033-017-4137-9

	Development of 13 microsatellite markers for Castanopsis tribuloides (Fagaceae) using next-generation sequencing
	Waikham, Pimubon 1; Thongkumkoon, Patcharawadee 1; Chomdej, Siriwadee 1; Liu, Aizhong2 ; Wangpakapattanawong, Prasit 1
	2018-02-01
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	45 期号:1 页码:27-30
摘要	Catanopsis tribuloides is a climax tree species commonly distributed in evergreen forests and has been used to restore degraded areas in northern Thailand. To aid in study of genetic diversity of the species, microsatellite markers, which are specific to C. tribuloides, were developed using whole genome sequencing by next-generation sequencing technology. The primers for microsatellite were developed and screened for optimal annealing temperature by PCR assay. The loci primers specific with C. tribuloides, 13 polymorphic microsatellite primers were successfully developed. The results from genetic information analyzing showed the number of alleles presented were between 2 and 24. Accordingly, the expected and observed heterozygosity obtained were between 0.298 and 0.920 and 0.364 to 1.000, respectively. Null allele frequency was presented 0.000-0.199. Genetic information was generated 10 loci primers significantly deviated from Hardy-Weinberg Equilibrium. All 13 primer pairs of loci were not significant with linkage disequilibrium. A set of microsatellite markers in this study could be applied to gene flow, genetic structure and population genetic studies in the future.
关键词	Castanopsis Tribuloides Forest Restoration Next-generation Sequencing Population Genetics Thailand
DOI	10.1007/s11033-017-4137-9
语种	英语
WOS记录号	WOS:000419919100004
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/60595
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chiang Mai Univ, Dept Biol, Fac Sci, 239 HuayKaew Rd, Chiang Mai 50200, Thailand 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Waikham, Pimubon,Thongkumkoon, Patcharawadee,Chomdej, Siriwadee,et al. Development of 13 microsatellite markers for Castanopsis tribuloides (Fagaceae) using next-generation sequencing[J]. MOLECULAR BIOLOGY REPORTS,2018,45(1):27-30.
APA	Waikham, Pimubon,Thongkumkoon, Patcharawadee,Chomdej, Siriwadee,Liu, Aizhong,&Wangpakapattanawong, Prasit.(2018).Development of 13 microsatellite markers for Castanopsis tribuloides (Fagaceae) using next-generation sequencing.MOLECULAR BIOLOGY REPORTS,45(1),27-30.
MLA	Waikham, Pimubon,et al."Development of 13 microsatellite markers for Castanopsis tribuloides (Fagaceae) using next-generation sequencing".MOLECULAR BIOLOGY REPORTS 45.1(2018):27-30.