Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis | |
Huang, Weiping; Lin, Yan; He, Mingxia; Gong, Yuhao; Huang, Junchao | |
2018-01-31 | |
发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
ISSN | 0021-8561 |
卷号 | 66期号:4页码:891-897 |
摘要 | Natural resources of zeaxanthin are extremely limited. A Chlorella zofingiensis mutant (CZ-bkt1), which could accumulate high amounts of zeaxanthin, was generated and characterized. CZ-bkt1 was achieved by treating the algal cells with a chemical mutagen followed by a color-based colony-screening approach. CZ-bkt1 was found to consist of a dysfunctional carotenoid ketolase, leading to the accumulation of zeaxanthin rather than to its downstream ketocarotenoid astaxanthin. Light irradiation, glucose, NaCl, and nitrogen deficiency all induced CZ-bkt1 to accumulate zeaxanthin. CZ-bkt1 accumulated zeaxanthin up to 7.00 +/- 0.82 mg/g when induced by high-light irradiation and nitrogen deficiency and up to 36.79 +/- 2.23 mg/L by additional feeding with glucose. Furthermore, in addition to zeaxanthin, CZ-bkt1 also accumulated high amounts of beta-carotene (7.18 +/- 0.72 mg/g or 34.64 +/- 1.39 mg/L) and lutein (13.81 +/- 1.23 mg/g or 33.97 +/- 2.61 mg/L). CZ-bkt1 is the sole species up to date with the ability to accumulate high amounts of the three carotenoids that are essential for human health. |
关键词 | Carotenoid Chlorella Zofingiensis Zeaxanthin Lutein Microalgae |
DOI | 10.1021/acs.jafc.7b05400 |
语种 | 英语 |
WOS记录号 | WOS:000424313300017 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/60565 |
专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
作者单位 | Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China |
推荐引用方式 GB/T 7714 | Huang, Weiping,Lin, Yan,He, Mingxia,et al. Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018,66(4):891-897. |
APA | Huang, Weiping,Lin, Yan,He, Mingxia,Gong, Yuhao,&Huang, Junchao.(2018).Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,66(4),891-897. |
MLA | Huang, Weiping,et al."Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 66.4(2018):891-897. |
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