Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis

doi:10.1021/acs.jafc.7b05400

	Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis
	Huang, Weiping; Lin, Yan; He, Mingxia; Gong, Yuhao; Huang, Junchao
	2018-01-31
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
卷号	66 期号:4 页码:891-897
摘要	Natural resources of zeaxanthin are extremely limited. A Chlorella zofingiensis mutant (CZ-bkt1), which could accumulate high amounts of zeaxanthin, was generated and characterized. CZ-bkt1 was achieved by treating the algal cells with a chemical mutagen followed by a color-based colony-screening approach. CZ-bkt1 was found to consist of a dysfunctional carotenoid ketolase, leading to the accumulation of zeaxanthin rather than to its downstream ketocarotenoid astaxanthin. Light irradiation, glucose, NaCl, and nitrogen deficiency all induced CZ-bkt1 to accumulate zeaxanthin. CZ-bkt1 accumulated zeaxanthin up to 7.00 +/- 0.82 mg/g when induced by high-light irradiation and nitrogen deficiency and up to 36.79 +/- 2.23 mg/L by additional feeding with glucose. Furthermore, in addition to zeaxanthin, CZ-bkt1 also accumulated high amounts of beta-carotene (7.18 +/- 0.72 mg/g or 34.64 +/- 1.39 mg/L) and lutein (13.81 +/- 1.23 mg/g or 33.97 +/- 2.61 mg/L). CZ-bkt1 is the sole species up to date with the ability to accumulate high amounts of the three carotenoids that are essential for human health.
关键词	Carotenoid Chlorella Zofingiensis Zeaxanthin Lutein Microalgae
DOI	10.1021/acs.jafc.7b05400
语种	英语
WOS记录号	WOS:000424313300017
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/60565
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Huang, Weiping,Lin, Yan,He, Mingxia,et al. Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2018,66(4):891-897.
APA	Huang, Weiping,Lin, Yan,He, Mingxia,Gong, Yuhao,&Huang, Junchao.(2018).Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,66(4),891-897.
MLA	Huang, Weiping,et al."Induced High-Yield Production of Zeaxanthin, Lutein, and beta-Carotene by a Mutant of Chlorella zofingiensis".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 66.4(2018):891-897.