Simplified and efficient DNA extraction protocol for Meliolaceae specimens
Zeng, Xiang-Yu1,2,3; Jeewon, Rajesh4; Wen, Ting-Chi1; Hongsanan, Sinang2; Boonmee, Saranyaphat2; Hyde, Kevin D.2,3,5
2018-03-01
发表期刊MYCOLOGICAL PROGRESS
ISSN1617-416X
卷号17期号:3页码:403-415
摘要Meliolaceae is an obligate biotrophic fungal family which cannot be cultured in artificial media. This has resulted in a lack of DNA sequence data in public databases to better resolve species taxonomy. The main criterion for specific classification, therefore, relied heavily on host association. Fresh collections from living leaves of Croton persimilis and Tamarindus indica with black colonies in Mae Fah Luang University, Thailand yielded a new species, Irenopsis crotonicola sp. nov., and a new record of Meliola tamarindi. These taxa are described and phenotypic comparisons are made with known species. To better classify the putative novel species that cannot be cultivated, we outline the protocol to extract DNA from fruiting bodies and generate new phylogenetic data. The results indicate that this direct DNA extraction method is suitable to yield quality DNA sufficient for polymerase chain reaction (PCR) amplifications of several commonly used DNA regions in fungal systematics. The 28S rDNA phylogram generated confirms the position of our taxa within Meliolaceae and indicate a close relationship of I. crotonicola sp. nov. to I. walsurae. Sequences of tef, beta-tubulin, and GPDH regions of Meliolaceae are provided as well.
关键词Black Mildews Meliolales Phylogeny Sordariomycetes
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/60562
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Guizhou Univ, Minist Educ, Engn Res Ctr Southwest Biopharmaceut Resources, Guiyang 550025, Guizhou, Peoples R China
2.Mae Fah Luang Univ, Ctr Excellence Fungal Res, Chiang Rai 57100, Thailand
3.Mae Fah Luang Univ, Sch Sci, Chiang Rai 57100, Thailand
4.Univ Mauritius, Dept Hlth Sci, Fac Sci, Reduit 80837, Mauritius
5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China
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Zeng, Xiang-Yu,Jeewon, Rajesh,Wen, Ting-Chi,et al. Simplified and efficient DNA extraction protocol for Meliolaceae specimens[J]. MYCOLOGICAL PROGRESS,2018,17(3):403-415.
APA Zeng, Xiang-Yu,Jeewon, Rajesh,Wen, Ting-Chi,Hongsanan, Sinang,Boonmee, Saranyaphat,&Hyde, Kevin D..(2018).Simplified and efficient DNA extraction protocol for Meliolaceae specimens.MYCOLOGICAL PROGRESS,17(3),403-415.
MLA Zeng, Xiang-Yu,et al."Simplified and efficient DNA extraction protocol for Meliolaceae specimens".MYCOLOGICAL PROGRESS 17.3(2018):403-415.
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