Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag

doi:10.1007/s00216-018-0872-6

	Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag
	Yu, Lingling 1,2; Wen, Chao 1,3; Li, Xing1,3 ; Fang, Shiqi 1,3; Yang, Lichuan 4; Wang, Tony 4; Hu, Kaifeng1
	2018-03-01
发表期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
卷号	410 期号:7 页码:2011-2018
摘要	Quantification of endogenous and exogenous plasma glucose can help more comprehensively evaluate the glucose metabolic status. A ratio-based approach using isotope dilution liquid chromatography tandem mass spectrometry (ID LC-MS/MS) with indirect multiple reaction monitoring (MRM) of the derivative tag was developed to simultaneously quantify endo-/exogenous plasma glucose. Using diluted D-[C-13(6)] glucose as tracer of exogenous glucose, C-12(6)/C-13(6) glucoses were first derivatized and then data were acquired in MRM mode. The metabolism of exogenous glucose can be tracked and the concentration ratio of endo/exo-genous glucose can be measured by calculating the endo-/exo-genous glucose concentrations from peak area ratio of specific daughter ions. Joint application of selective derivatization and MRM analysis not only improves the sensitivity but also minimizes the interference from the background of plasma, which warrants the accuracy and reproducibility. Good agreement between the theoretical and calculated concentration ratios was obtained with a linear correlation coefficient (R) of 0.9969 in the range of D-glucose from 0.5 to 20.0 mM, which covers the healthy and diabetic physiological scenarios. Satisfactory reproducibility was obtained by evaluation of the intra- and inter-day precisions with relative standard deviations (RSDs) less than 5.16%, and relative recoveries of 85.96 to 95.92% were obtained at low, medium, and high concentration, respectively. The method was successfully applied to simultaneous determination of the endo-/exogenous glucose concentration in plasma of non-diabetic and type II diabetic cynomolgus monkeys.
关键词	Derivatization Diabetes Endogenous Glucose Exogenous Glucose Lc-ms/ms
DOI	10.1007/s00216-018-0872-6
语种	英语
WOS记录号	WOS:000425525400016
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/60547
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Kunming Biomed Int, Kunming 650500, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yu, Lingling,Wen, Chao,Li, Xing,et al. Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(7):2011-2018.
APA	Yu, Lingling.,Wen, Chao.,Li, Xing.,Fang, Shiqi.,Yang, Lichuan.,...&Hu, Kaifeng.(2018).Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(7),2011-2018.
MLA	Yu, Lingling,et al."Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.7(2018):2011-2018.