Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag
Yu, Lingling1,2; Wen, Chao1,3; Li, Xing1,3; Fang, Shiqi1,3; Yang, Lichuan4; Wang, Tony4; Hu, Kaifeng1
2018-03-01
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
Volume410Issue:7Pages:2011-2018
AbstractQuantification of endogenous and exogenous plasma glucose can help more comprehensively evaluate the glucose metabolic status. A ratio-based approach using isotope dilution liquid chromatography tandem mass spectrometry (ID LC-MS/MS) with indirect multiple reaction monitoring (MRM) of the derivative tag was developed to simultaneously quantify endo-/exogenous plasma glucose. Using diluted D-[C-13(6)] glucose as tracer of exogenous glucose, C-12(6)/C-13(6) glucoses were first derivatized and then data were acquired in MRM mode. The metabolism of exogenous glucose can be tracked and the concentration ratio of endo/exo-genous glucose can be measured by calculating the endo-/exo-genous glucose concentrations from peak area ratio of specific daughter ions. Joint application of selective derivatization and MRM analysis not only improves the sensitivity but also minimizes the interference from the background of plasma, which warrants the accuracy and reproducibility. Good agreement between the theoretical and calculated concentration ratios was obtained with a linear correlation coefficient (R) of 0.9969 in the range of D-glucose from 0.5 to 20.0 mM, which covers the healthy and diabetic physiological scenarios. Satisfactory reproducibility was obtained by evaluation of the intra- and inter-day precisions with relative standard deviations (RSDs) less than 5.16%, and relative recoveries of 85.96 to 95.92% were obtained at low, medium, and high concentration, respectively. The method was successfully applied to simultaneous determination of the endo-/exogenous glucose concentration in plasma of non-diabetic and type II diabetic cynomolgus monkeys.
KeywordDerivatization Diabetes Endogenous Glucose Exogenous Glucose Lc-ms/ms
DOI10.1007/s00216-018-0872-6
Language英语
WOS IDWOS:000425525400016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/60547
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Yunnan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Kunming Biomed Int, Kunming 650500, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Yu, Lingling,Wen, Chao,Li, Xing,et al. Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(7):2011-2018.
APA Yu, Lingling.,Wen, Chao.,Li, Xing.,Fang, Shiqi.,Yang, Lichuan.,...&Hu, Kaifeng.(2018).Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(7),2011-2018.
MLA Yu, Lingling,et al."Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.7(2018):2011-2018.
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