Phospholipase D1-mediated phosphatidic acid change is a key determinant of desiccation-induced viability loss in seeds
Chen, Hongying1,2; Yu, Xiaomei2; Zhang, Xudong2; Yang, Lan2; Huang, Xing2,4; Zhang, Jie2; Pritchard, Hugh W.3; Li, Weiqi1,2
2018
Source PublicationPLANT CELL AND ENVIRONMENT
ISSN0140-7791
Volume41Issue:1Pages:50-63
AbstractHigh sensitivity of seeds to water loss is a widespread phenomenon in the world's plant species. The molecular basis of this trait is poorly understood but thought to be associated with critical changes in membrane function. We profiled membrane lipids of seeds in eight species with varying levels of desiccation tolerance and found a close association between reducing seed viability and increasing phosphatidic acid (PA). We applied hydration-dehydration cycles to Arabidopsis seeds, which are normally desiccation tolerant, to mimic the onset of desiccation sensitivity with progression towards germination and examined the role of phospholipase D (PLD) in desiccation stress-induced production of PA. We found that PLD1 became more abundant and migrated from the cytosol to the membrane during desiccation, whereas PLD did not change, and that all desiccation-induced PA was derived from PLD1 hydrolysis. When PLD1 was suppressed, the germination level after each hydration-dehydration cycle improved significantly. We further demonstrated that PLD1-mediated PA formation modulates desiccation sensitivity as applying its inhibitor improved seed desiccation tolerance and its suppression in protoplasts enhanced survival under dehydration. The insights provided by comparative lipidomics enable us to propose a new membrane-based model for seed desiccation stress and survival.
KeywordDrought Membrane Lipid Phosphatidic Acid (Pa) Phospholipase d (Pld) Seed Storage Behaviour Water Relation
DOI10.1111/pce.12925
Language英语
WOS IDWOS:000418419500004
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/60476
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China
3.Royal Bot Gardens, Wakehurst Pl, Richmond RH17 6TN, W Sussex, England
4.Yunnan Agr Univ, Dept Phytopathol, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Hongying,Yu, Xiaomei,Zhang, Xudong,et al. Phospholipase D1-mediated phosphatidic acid change is a key determinant of desiccation-induced viability loss in seeds[J]. PLANT CELL AND ENVIRONMENT,2018,41(1):50-63.
APA Chen, Hongying.,Yu, Xiaomei.,Zhang, Xudong.,Yang, Lan.,Huang, Xing.,...&Li, Weiqi.(2018).Phospholipase D1-mediated phosphatidic acid change is a key determinant of desiccation-induced viability loss in seeds.PLANT CELL AND ENVIRONMENT,41(1),50-63.
MLA Chen, Hongying,et al."Phospholipase D1-mediated phosphatidic acid change is a key determinant of desiccation-induced viability loss in seeds".PLANT CELL AND ENVIRONMENT 41.1(2018):50-63.
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