Leaves, not roots or floral tissue, are the main site of rapid, external pressure-induced ABA biosynthesis in angiosperms

doi:10.1093/jxb/erx480

	Leaves, not roots or floral tissue, are the main site of rapid, external pressure-induced ABA biosynthesis in angiosperms
	Zhang, Feng-Ping 1; Sussmilch, Frances 2,3; Nichols, David S.4; Cardoso, Amanda A.3,5; Brodribb, Timothy J.3; McAdam, Scott A. M.6,7
	2018-02-20
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	69 期号:5 页码:1261-1267
摘要	Rapid biosynthesis of abscisic acid (ABA) in the leaf, triggered by a decrease in cell volume, is essential for a functional stomatal response. However, it is not known whether rapid biosynthesis of ABA is also triggered in other plant tissues. Through the application of external pressure to flower, root, and leaf tissues, we test whether a reduction in cell volume can trigger rapid increases in ABA levels across the plant body in two species, Solanum lycopersicum and Passiflora tarminiana. Our results show that, in contrast to rapid ABA synthesis in the leaf, flower and root tissue did not show a significant, increase in ABA level in response to a drop in cell volume over a short time frame, suggesting that rapid ABA biosynthesis occurs only in leaf, not in flower or root tissues. A gene encoding the key, rate-limiting carotenoid cleavage enzyme (9-cis-epoxycarotenoid dioxygenase, NCED) in the ABA biosynthetic pathway in S. lycopersicum, NCED1, was upregulated to a lesser degree in flowers and roots compared with leaves in response to applied pressure. In both species, floral tissues contained substantially lower levels of the NCED substrate 9'-cis-neoxanthin than leaves, and this ABA precursor could not be detected in roots. Slow and minimal ABA biosynthesis was detected after 2 h in petals, indicating that floral tissue is capable of synthesizing ABA in response to sustained water deficit. Our results indicate that rapid ABA biosynthesis predominantly occurs in the leaves, and not in other tissues.
关键词	Abscisic Acid Cell Volume 9 '-cis-epoxycarotenoid Dioxygenase Flowers Roots Stomata
DOI	10.1093/jxb/erx480
语种	英语
WOS记录号	WOS:000426147200027
引用统计	被引频次：78[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/60447
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Univ Wurzburg, Inst Mol Plant Physiol & Biophys, D-97082 Wurzburg, Germany 3.Univ Tasmania, Sch Biol Sci, Hobart, Tas 7001, Australia 4.Univ Tasmania, Cent Sci Lab, Hobart, Tas 7001, Australia 5.Univ Fed Vicosa, Dept Biol Vegetal, Campus Univ, Vicosa, MG, Brazil 6.Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47906 USA 7.Purdue Univ, Purdue Ctr Plant Biol, W Lafayette, IN 47906 USA
推荐引用方式 GB/T 7714	Zhang, Feng-Ping,Sussmilch, Frances,Nichols, David S.,et al. Leaves, not roots or floral tissue, are the main site of rapid, external pressure-induced ABA biosynthesis in angiosperms[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(5):1261-1267.
APA	Zhang, Feng-Ping,Sussmilch, Frances,Nichols, David S.,Cardoso, Amanda A.,Brodribb, Timothy J.,&McAdam, Scott A. M..(2018).Leaves, not roots or floral tissue, are the main site of rapid, external pressure-induced ABA biosynthesis in angiosperms.JOURNAL OF EXPERIMENTAL BOTANY,69(5),1261-1267.
MLA	Zhang, Feng-Ping,et al."Leaves, not roots or floral tissue, are the main site of rapid, external pressure-induced ABA biosynthesis in angiosperms".JOURNAL OF EXPERIMENTAL BOTANY 69.5(2018):1261-1267.