1.以“生脉注射液”初步证明中药复方是多靶作用机理的组合天然化学库;2.三种植物的化学成分研究
其他题名1. Preliminary proving that compound prescription of TCM is a combinatorial natural chemical library acted as multitarget with "sheng mai san" injection 2. study on the chemical constituents of three plants
戴好富
学位类型博士
导师周俊
2001
学位授予单位中国科学院昆明植物研究所
学位授予地点中国科学院昆明植物研究所
关键词中药复方 物质基础 作用机理 生脉注射液 化学成分
摘要该论文共分为三章.第一章以生脉注射液为对象进行研究,分别对组成"生脉注射液,,的4个组成部分:红参(PanaxginsengC.A.Meyer)的乙醇提取物、麦冬(Ophiopogojaponicus(Thunb.)Ker-Gawl.)的水提醇沉物、北五味子(Schisandrachinensis(Turcz.)Bail1.)的挥发油和水提醇沉物的化学和初步药理进行了综合研究.第二章共二节,分别介绍了对石竹科两种植物狗筋蔓(CucubalusbacciferL.)和毛剪秋罗(Lychniscoronaria(L.)Desr.),旋花科植物七爪龙(IpomoeadigitataLinn.)的化学成分研究情况.第三章综述了木兰科五味子族植物中非木脂素类化学成分的研究进展.
其他摘要The thesis is mainly composed of three chapters. The first chapter including three sections is about study on compound prescription "Sheng Mai San" injection, which was composed of ethanolic extract of Panax ginseng, the EtOH precipitate of water extract of Ophiopogon japonicus, the EtOH precipitate of water extract and volatile oil of Schisandra chinensis. After the Chemical and Pharmacological study on "Sheng Mai San" injection, the results showed that the substance base of "Sheng Mai San" injection including the natural products of ginsenosides, steriodal glycosides from Ophiopogon japonicus, volatile constituents and organic acid from Schisandra chinensis, etc. The targets of "Sheng Mai San" injection in the body including Calcium channel, AC, NOS, SOD and NMDA etc. So it can be concluded that the substance base of "Sheng Mai San" injection is Combinatorial Natural Chemical Library (CNCL), and the mechanism of action is multitarget. As a part of preliminary work of fingerprints of "Sheng Mai San" injection, we mapped the HPLC/MS fingerprints of ethanolic extract of Panax ginseng and the EtOH precipitate of water extract of Ophiopogon japonicus, the HPLC fingerprint of the EtOH precipitate of water extract of Schisandra chinensis and the GC/MS fingerprint of volatile oil of Schisandra chinensis. 82 compounds including 50 volatile constituents have been identified from O. japonicus and S. chinensis, six of them are new ones. Ophiopogon japonicus belongs to Liliaceae, which is distributed in Mianyang of Sichuan province and Hangzhou, Yuyao, Xiaoshan of Zhejiang province. The tuber of O.japonicus is a traditional Chinese medicine, which is recorded to have various functions, such as against cardiovascular diseases and anti-bacteria, and used as a potent drug to treat different diseases, especially heart and lung diseases for a long time. Since the first steriodal glycoside was isolated from the plant by Japanese scholars, much attention has been paid to studies of the chemical components of 0. japonicus in recent decades. Steroidal glycosides, flavones and monoterpenoid glycosides have been isolated from this medicinal plant. During our reinvestigation of the n-BuOH extract of 0. japonicus, 15 compounds were identified as Ophiopojaponin A (1), Ophiopojaponin B (2), Ophiopojaponin C (3), Ophiopojaponin D (4), L-borneol O-P-D-glucopyranoside (5), L-borneol O-P-D- apiofuranosyl (l-"6)-O-P-D-glucopyranoside (6), 4-allyl-l, 2-benzenediol 1-O-a-L- rhamnopyranosyl (l-"6)-O-p-D-gmcopyranoside (7), (22S)-cholest-5-ene- 1P, 3p, 16p, 22-tetrol 1-0-a-L-rhamnopyranosyl 16-0-P-D-glucopyranoside (8), Ophiopogonin D (9), Ophiopogenin D' (10), Diosgenin 3-O-[2-O-acetyl-a-L-rham- nopyranosyl (l-"2)]-P-D-xylopyranosyl (l->3)-p-D-glucopyranoside (11), Ruscogenin l-O-[2-O-acetyl-a-L-rhamnopyranosyl (l-"2)]-p-D-xylopyranosyl (l->3)-P-D-fucopyranoside (12), 1-O-P-D-fructofuranosyl-P-D-fractopyranoside (13), L-pyroglutamic acid (14), Daucosterol (15). Compound 1-4 are new ones, and compound 7,8,14 were firstly isolated from O.japonicus. The fruits of Schisandra chinensis is a traditional Chinese medicine which is uesed to cure pulmonary diseases and liver diseases. It's reported that more than 30 ligans were isolated from S. chinensis. The volatile oil of S. chinensis showed strong anti-PAI bioactivity tested in Lab for screening of Laboratory of Phytochemistry (Kib, CAS), and 50 volatile constituents were elucidated by GC/MS analysis. After bioassay by Prof. Yiping Wang in SIMM (CAS), the water extract of S. showed cardiovascular activity including reducing heart rhytlim, inhibiting platelet aggregation and vasodilation. 28 compounds were isolated from S. chinensis, and 19 of them were elucidated as Schisanchinin A (1), Schisanchinin B (2), Protocatechuic acid (3), Quinic acid (4), 2-Methyl citrate (5), 5-Hydroxymethyl-2-furancarboxalde-hyde (6), Zingerone glucoside (7), Thymoquinol 2-glucoside (8), Thymoquinol 5-glucoside (9), Benzyl-P-D-glucopyranosyl(2-"l)-|3-D-glucopyranoside (10), Benzyl-p-D-glucopyranosyl(6->l)-p-D-glucopyranoside (11), (+)-Isoscoparin (12), Quercetin 3-O-Rutinoside (13), p-D-glucopyranosyl(l-"4) p-D-mannopyrsnoside (14), Malic acid 1-Et ester (15), Heptaacetyl-aralia cerebroside (16), Hexaacetyl-soya cerebroside II (17), L-pyroglutamic acid (18), Daucosterol (19). Compound 1 and 2 are new ones, and compound 3-17 were firstly isolated from this plant. The second chapter involving two sections deals with chemical constituents from Cucubalus baccifer, Lychnis coronaria and Ipomoea digitata. 29 compounds were elucidated from the former plants, and two of them are new ones. Lychnis coronaria belongs to Caryophyllaceae which is distributed in south of Europe and west of Asia. 15 compounds were isolated from the whole plant of L. coronaria. They are identified as Tricin 7-O-glucopyranoside (1), (+)-Isoscoparin (2), Epoxyactinidionoside (3), la, 20R-Hydroxyecdysone (4), Ecdysterone (5), Polypodine B (6), Ecdysterone 22-O-P-D-glucopyranoside (7), Stigmast-5-ene-3-one (8), Taraxerol (9), a-tocopherol (10), 10-eicosyl alcohol (11), Nerol (12), Dehydrodiconiferyl alcohol 4-O-P-D-glucopyranoside (13), |3-Stiosterol (14), Daucosterol (15), which are all isolated for the first time from L. coronaria. As a continuous study of cucubalus baccifer, structures of three compounds in our group work were elucidated as Baccifer acid (1), Baccifine A (2), Isovitexin (3). Compounds 1 and 2 are new ones. Ipomoea digitata belongs to Convolvulaceae, which distributed in Yunnan, Guangdong, Guangxi and Taiwan province. It's considered to be poisonous in folk, and used to cure hydropsy and carbuncle. During the course of phytochemical investigation on the ethanol extract of the tubers of I. digitata led to the isolation of 13 compounds, their structures were determined as Taraxerol (1), Taraxerol actate (2), Umbelliferone (3), Octadecyl (E)-p-coumarate (4), Palmitic acid-2,3-dihydroxy-propanenyl ester (5), Scopoletin (6) , Scopolin (7), n-Butyl-f3-D-fructopyranoside (8), Phenylalanine (9), fi-Sitosterol 3-O-p-D-galactopyranoside (10), Caffeic acid (11), (5-Sitosterol(12),Daucosterol(13)o Compounds 4, 5, 7-11 were isolated from /. digitata for the first time. Finally, progress of non-lignan chemical constituents isolated from Shisandreae during past years has been reviewed in the third chapter.
页数126
语种中文
文献类型学位论文
条目标识符http://ir.kib.ac.cn/handle/151853/578
专题昆明植物所硕博研究生毕业学位论文
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戴好富. 1.以“生脉注射液”初步证明中药复方是多靶作用机理的组合天然化学库;2.三种植物的化学成分研究[D]. 中国科学院昆明植物研究所. 中国科学院昆明植物研究所,2001.
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