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Characterization of tailoring genes involved in the modification of geldanamycin polyketide
Shin, JC ; Na, Z ; Lee, D ; Kim, W ; Lee, K ; Shen, YM ; Paik, SG ; Hong, YS ; Lee, JJ
2008
发表期刊JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN1017-7825
卷号18期号:6页码:1101-1108
摘要Geldanamycin and its analogs are important anticancer agents that inhibit the newly targeted heat-shock protein (Hsp) 90, which is a chaperone protein in eukaryotic cells. To resolve which geldanamycin biosynthetic genes are responsible for particular post-polyketide synthase (PKS) processing steps and in which order the reactions occur, we individually inactivated candidate genes in Streptomyces hygroscopicus subsp. duamyceticus JCM4427 and isolated and elucidated the structures of intermediates from each mutant. The results indicated that gel7 governs at least one of the benzoquinone ring oxidation steps. The gel16 was found to be involved in double-bond formation between C-4 and C-5 of 4,5-dihydrogeldanamycin, which confirmed our previous findings that this double bond is reduced during the post-PKS modification of the polyketide assembly. In addition, pro-geldanamycin, which does not possess a double bond at C-4/5, was purified from the gel7 and gel8 double-gene-inactivated mutant.
关键词Geldanamycin Biosynthesis Streptomyces Polyketide
收录类别sci
语种英语
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/5603
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Shin, JC,Na, Z,Lee, D,et al. Characterization of tailoring genes involved in the modification of geldanamycin polyketide[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2008,18(6):1101-1108.
APA Shin, JC.,Na, Z.,Lee, D.,Kim, W.,Lee, K.,...&Lee, JJ.(2008).Characterization of tailoring genes involved in the modification of geldanamycin polyketide.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,18(6),1101-1108.
MLA Shin, JC,et al."Characterization of tailoring genes involved in the modification of geldanamycin polyketide".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 18.6(2008):1101-1108.
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