Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study

doi:10.1016/j.jep.2017.01.026

	Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study
	Wu,Jie; li,Xue; Wan,Wencheng; Yang,Qiaohong; Ma,Weifeng; Chen,Dan; Hu,Jiangmiao; Chen,C. -Y. Oliver; Wei,Xiaoyong
	2017
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	0378-8741
卷号	198 页码:255-261
摘要	Ethnopharmacological relevance: Dendrobium. chrysotoxum Lindl is a commonly used species of medicinal Dendrobium which belongs to the family of Orchidaceae, locally known as "Shihu" or "Huangcao". D. chrysotoxum Lindl is widely known for medicinal values in traditional Chinese medicine as it possesses anti-inflammatory, anti-hyperglycemic induction, antitumor and antioxidant properties. Study aim: To characterize the interaction between gigantol extracted from D. chrysotoxum Lindl and the AR gene, and determine gigantol's efficacy against cataractogenesis. Materials and methods: Human lens epithelial cells (HLECs) were induced by glucose as the model group. Reverse transcription polymerase chain reaction (RT-PCR) was used to assess AR gene expression. Then, the mode of interaction of gigantol with the AR gene was evaluated by UV-visible spectroscopy, atomic force microscope (AFM) and surface-enhanced Raman spectroscopy (SERS). The binding constant was determined by UV-visible. Results: Gigantol depressed AR gene expression in HLECs. UV visible spectra preliminarily indicated that interaction between the AR gene and gigantol may follow the groove mode, with a binding constant of 1.85x10(3)L/mol. Atomic force microscope (AFM) data indicated that gigantol possibly bound to insert AR gene base pairs of the double helix. Surface-enhanced Raman spectroscopy (SERS) studies further supported these observations. Conclusion: Gigantol extracted from D. chrysotoxum Lindl not only has inhibitory effects on aldose reductase, but also inhibits AR gene expression. These findings provide a more comprehensive theoretical basis for the use of Dendrobium for the treatment of diabetic cataract.
关键词	Enhanced Raman-scattering Calf Thymus Dna Diabetic Complications Cataract Rats Projections Progression Prevalence Blindness Cells Gigantol Aldose Reductase (Ar) Gene Interaction Uv-visible Spectroscopy Atomic Force Microscopy (Afm) Surface-enhanced Raman Spectroscopy (Sers)
学科领域	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
DOI	10.1016/j.jep.2017.01.026
收录类别	SCI
语种	英语
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/54962
专题	植物化学与西部植物资源持续利用国家重点实验室
推荐引用方式 GB/T 7714	Wu,Jie,li,Xue,Wan,Wencheng,et al. Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study[J]. JOURNAL OF ETHNOPHARMACOLOGY,2017,198:255-261.
APA	Wu,Jie.,li,Xue.,Wan,Wencheng.,Yang,Qiaohong.,Ma,Weifeng.,...&Wei,Xiaoyong.(2017).Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study.JOURNAL OF ETHNOPHARMACOLOGY,198,255-261.
MLA	Wu,Jie,et al."Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study".JOURNAL OF ETHNOPHARMACOLOGY 198(2017):255-261.

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