Structural analysis of a homogeneous polysaccharide from Achatina fulica

doi:10.1016/j.ijbiomac.2017.01.149

	Structural analysis of a homogeneous polysaccharide from Achatina fulica
	Liu,Jie; Shang,Feineng; Yang,Zengming; Wu,Mingyi; Zhao,Jinhua
	2017
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	98 页码:786-792
摘要	Edible snails have been widely used as a health food and medicine in many countries. In our study, a water-soluble polysaccharide (AF-1) was isolated and purified from Achatina fulica by papain enzymolysis, alcohol precipitation and strong anion exchange chromatography. Structureof the polysaccharide was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, high performance liquid chromatography, analysis of monosaccharide composition, methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy (H-1,C-13, COSY, TOCSY, NOESY, HSQC and HMBC). Chemical composition analysis indicated that AF-1 is composed of glucose (Glc) and its average molecular weight is 1710 kDa. Structural analysis suggested that AF-1 is mainly consisted of a linear repeating backbone of (1 -> 4) linked alpha-D-Glc p residues with one branch, a-D-Glc p, attached to the main chain by (1 -> 6) glycosidic bonds at every five main-chain units. Further studies on biological activities of the polysaccharide are currently in progress. (C) 2017 Elsevier B.V. All rights reserved.
关键词	Anticoagulant Activities Acharan Sulfate Sea-cucumber Glycosaminoglycan Chromatography Spectroscopy Derivatives Snail Polysaccharide Chemical Structure Nmr
学科领域	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
DOI	10.1016/j.ijbiomac.2017.01.149
收录类别	SCI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/54953
专题	植物化学与西部植物资源持续利用国家重点实验室
推荐引用方式 GB/T 7714	Liu,Jie,Shang,Feineng,Yang,Zengming,et al. Structural analysis of a homogeneous polysaccharide from Achatina fulica[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,98:786-792.
APA	Liu,Jie,Shang,Feineng,Yang,Zengming,Wu,Mingyi,&Zhao,Jinhua.(2017).Structural analysis of a homogeneous polysaccharide from Achatina fulica.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,98,786-792.
MLA	Liu,Jie,et al."Structural analysis of a homogeneous polysaccharide from Achatina fulica".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 98(2017):786-792.

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