| Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N-tabacum | |
Chen, Yongdui1,2,3; Dong, Jiahong1,2,3; Bennetzen, Jeffrey L.4; Zhong, Micai4; Yang, Jun2,5; Zhang, Jie1,3; Li, Shunlin6; Hao, Xiaojiang4,6 ; Zhang, Zhongkai1,2,3; Wang, Xuewen4,7
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| 2017-10-02 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 7Pages:12504 |
| Abstract | 3-Acetonyl-3-hydroxyoxindole (AHO) induces systemic acquired resistance (SAR) in Nicotiana. However, the underlying molecular mechanism is not well understood. To understand the molecular regulation during SAR induction, we examined mRNA levels, microRNA (miRNA) expression, and their regulatory mechanisms in control and AHO-treated tobacco leaves. Using RNA-seq analysis, we identified 1,445 significantly differentially expressed genes (DEGs) at least 2 folds with AHO treatment. The DEGs significantly enriched in six metabolism pathways including phenylpropanoid biosynthesis, sesquiterpenoid and triterpenoid biosynthesis for protective cuticle and wax. Key DEGs including PALs and PR-10 in salicylic acid pathway involved in SAR were significantly regulated. In addition, we identified 403 miRNAs belonging to 200 miRNA families by miRNA sequencing. In total, AHO treatment led to 17 up-and 6 down-regulated at least 2 folds (Wald test, P < 0.05) miRNAs (DEMs), respectively. Targeting analysis implicated four DEMs regulating three DEGs involved in disease resistance, including miR156, miR172f, miR172g, miR408a, SPL6 and AP2. We concluded that both mRNA and miRNA regulation enhances AHO-induced SAR. These data regarding DEGs, miRNAs, and their regulatory mechanisms provide molecular evidence for the mechanisms involved in tobacco SAR, which are likely to be present in other plants. |
| Keyword | Plant Innate Immunity Acibenzolar-s-methyl Flue-cured Tobacco Salicylic-acid Small Rnas Stress Responses Expression Arabidopsis Target Mirna |
| Subject Area | Multidisciplinary Sciences |
| DOI | 10.1038/s41598-017-12249-y |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000412050100025 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/54874 |
| Collection | 植物化学与西部植物资源持续利用国家重点实验室 |
| Affiliation | 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650223, Yunnan, Peoples R China 2.Minist Agr, Yunnan Prov Key Lab Agr Biotechnol, Kunming 650223, Yunnan, Peoples R China 3.Minist Agr, Key Lab Southwestern Crop Gene Resource & Germpla, Kunming 650223, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 5.Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou 450001, Henan, Peoples R China 6.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 7.Univ Georgia, Dept Genet, Athens, GA 30602 USA |
| Recommended Citation GB/T 7714 | Chen, Yongdui,Dong, Jiahong,Bennetzen, Jeffrey L.,et al. Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N-tabacum[J]. SCIENTIFIC REPORTS,2017,7:12504. |
| APA | Chen, Yongdui.,Dong, Jiahong.,Bennetzen, Jeffrey L..,Zhong, Micai.,Yang, Jun.,...&Wang, Xuewen.(2017).Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N-tabacum.SCIENTIFIC REPORTS,7,12504. |
| MLA | Chen, Yongdui,et al."Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N-tabacum".SCIENTIFIC REPORTS 7(2017):12504. |
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| 10.1038@s41598-017-1（3093KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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