Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N-tabacum
Chen, Yongdui1,2,3; Dong, Jiahong1,2,3; Bennetzen, Jeffrey L.4; Zhong, Micai4; Yang, Jun2,5; Zhang, Jie1,3; Li, Shunlin6; Hao, Xiaojiang4,6; Zhang, Zhongkai1,2,3; Wang, Xuewen4,7
2017-10-02
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7Pages:12504
Abstract3-Acetonyl-3-hydroxyoxindole (AHO) induces systemic acquired resistance (SAR) in Nicotiana. However, the underlying molecular mechanism is not well understood. To understand the molecular regulation during SAR induction, we examined mRNA levels, microRNA (miRNA) expression, and their regulatory mechanisms in control and AHO-treated tobacco leaves. Using RNA-seq analysis, we identified 1,445 significantly differentially expressed genes (DEGs) at least 2 folds with AHO treatment. The DEGs significantly enriched in six metabolism pathways including phenylpropanoid biosynthesis, sesquiterpenoid and triterpenoid biosynthesis for protective cuticle and wax. Key DEGs including PALs and PR-10 in salicylic acid pathway involved in SAR were significantly regulated. In addition, we identified 403 miRNAs belonging to 200 miRNA families by miRNA sequencing. In total, AHO treatment led to 17 up-and 6 down-regulated at least 2 folds (Wald test, P < 0.05) miRNAs (DEMs), respectively. Targeting analysis implicated four DEMs regulating three DEGs involved in disease resistance, including miR156, miR172f, miR172g, miR408a, SPL6 and AP2. We concluded that both mRNA and miRNA regulation enhances AHO-induced SAR. These data regarding DEGs, miRNAs, and their regulatory mechanisms provide molecular evidence for the mechanisms involved in tobacco SAR, which are likely to be present in other plants.
KeywordPlant Innate Immunity Acibenzolar-s-methyl Flue-cured Tobacco Salicylic-acid Small Rnas Stress Responses Expression Arabidopsis Target Mirna
Subject AreaMultidisciplinary Sciences
DOI10.1038/s41598-017-12249-y
Indexed BySCI
Language英语
WOS IDWOS:000412050100025
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/54874
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650223, Yunnan, Peoples R China
2.Minist Agr, Yunnan Prov Key Lab Agr Biotechnol, Kunming 650223, Yunnan, Peoples R China
3.Minist Agr, Key Lab Southwestern Crop Gene Resource & Germpla, Kunming 650223, Yunnan, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China
5.Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhengzhou 450001, Henan, Peoples R China
6.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
7.Univ Georgia, Dept Genet, Athens, GA 30602 USA
Recommended Citation
GB/T 7714
Chen, Yongdui,Dong, Jiahong,Bennetzen, Jeffrey L.,et al. Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N-tabacum[J]. SCIENTIFIC REPORTS,2017,7:12504.
APA Chen, Yongdui.,Dong, Jiahong.,Bennetzen, Jeffrey L..,Zhong, Micai.,Yang, Jun.,...&Wang, Xuewen.(2017).Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N-tabacum.SCIENTIFIC REPORTS,7,12504.
MLA Chen, Yongdui,et al."Integrating transcriptome and microRNA analysis identifies genes and microRNAs for AHO-induced systemic acquired resistance in N-tabacum".SCIENTIFIC REPORTS 7(2017):12504.
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