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Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing
Kranner, Ilse1; Chen, Hongying1,2; Pritchard, Hugh W.1; Pearce, Stephen R.3; Birtic, Simona1,3
2011
发表期刊PLANT GROWTH REGULATION
ISSN0167-6903
卷号63期号:1页码:63-72
摘要The germination of viable seeds is the basis for new plant growth and development. Seeds lose viability during storage, but the biochemical mechanisms of seed death are not fully understood. This study aimed to investigate degradation patterns of nucleic acids during seed ageing and subsequent water uptake. Seeds of Pisum sativum L. were artificially aged at 50A degrees C and 12% seed water content (WC). Nucleic acids degradation was studied during ageing and during imbibition of four seed lots with differential viability from highly viable to dead. As seeds lost viability during ageing, DNA was gradually degraded into internucleosomal fragments, resulting in 'DNA laddering', in conjunction with disintegration of 18S and 28S rRNA bands. During imbibition, non-aged controls had high levels of DNA and RNA integrity through to radicle protrusion. In an aged seed lot with 85% total germination (TG) DNA fragmentation decreased upon imbibition probably due to nucleosome degradation, while rRNA integrity did not improve. In an aged seed lot with 44% TG, neither DNA nor rRNA integrity improved upon imbibition. Dead seeds showed DNA degradation as laddering throughout imbibition along with extensive degradation of rRNA. We present a model in which interlinked programmed and non-programmed events contribute to seed ageing, and suggest that protection of nucleic acids during ageing is key to seed longevity.
关键词Ageing Dna Pisum Sativum Programmed Cell Death Rna Seed
资助信息Millennium Commission; Welcome Trust; Orange Plc.; Defra
学科领域Plant Sciences
DOI10.1007/s10725-010-9512-7
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000285365700008
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/5203
专题中国西南野生生物种质资源库
作者单位1.Royal Bot Gardens, Seed Conservat Dept, Richmond RH17 6TN, W Sussex, England
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 661100, Peoples R China
3.Univ Sussex, Sch Life Sci, Brighton BN1 9QG, E Sussex, England
推荐引用方式
GB/T 7714
Kranner, Ilse,Chen, Hongying,Pritchard, Hugh W.,et al. Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing[J]. PLANT GROWTH REGULATION,2011,63(1):63-72.
APA Kranner, Ilse,Chen, Hongying,Pritchard, Hugh W.,Pearce, Stephen R.,&Birtic, Simona.(2011).Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing.PLANT GROWTH REGULATION,63(1),63-72.
MLA Kranner, Ilse,et al."Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing".PLANT GROWTH REGULATION 63.1(2011):63-72.
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