中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 中国西南野生生物种质资源库  > 期刊论文
题名: Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing
作者: Kranner, Ilse1; Chen, Hongying1, 2; Pritchard, Hugh W.1; Pearce, Stephen R.3; Birtic, Simona1, 3
刊名: PLANT GROWTH REGULATION
关键词: Ageing DNA ; Pisum sativum ; Programmed cell death ; RNA ; Seed
英文摘要: The germination of viable seeds is the basis for new plant growth and development. Seeds lose viability during storage, but the biochemical mechanisms of seed death are not fully understood. This study aimed to investigate degradation patterns of nucleic acids during seed ageing and subsequent water uptake. Seeds of Pisum sativum L. were artificially aged at 50A degrees C and 12% seed water content (WC). Nucleic acids degradation was studied during ageing and during imbibition of four seed lots with differential viability from highly viable to dead. As seeds lost viability during ageing, DNA was gradually degraded into internucleosomal fragments, resulting in 'DNA laddering', in conjunction with disintegration of 18S and 28S rRNA bands. During imbibition, non-aged controls had high levels of DNA and RNA integrity through to radicle protrusion. In an aged seed lot with 85% total germination (TG) DNA fragmentation decreased upon imbibition probably due to nucleosome degradation, while rRNA integrity did not improve. In an aged seed lot with 44% TG, neither DNA nor rRNA integrity improved upon imbibition. Dead seeds showed DNA degradation as laddering throughout imbibition along with extensive degradation of rRNA. We present a model in which interlinked programmed and non-programmed events contribute to seed ageing, and suggest that protection of nucleic acids during ageing is key to seed longevity.
出版日期: 2011
卷号: 63, 期号:1, 页码:63-72
语种: 英语
收录类别: SCI
学科分类: Plant Sciences
ISSN号: 0167-6903
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/5203
Appears in Collections:中国西南野生生物种质资源库_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
201204090009.pdf(418KB)----开放获取--View Download

作者单位: 1.Royal Bot Gardens, Seed Conservat Dept, Richmond RH17 6TN, W Sussex, England
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 661100, Peoples R China
3.Univ Sussex, Sch Life Sci, Brighton BN1 9QG, E Sussex, England

Recommended Citation:
Kranner, I; Chen, HY; Pritchard, HW; Pearce, SR; Birtic, S.Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing,PLANT GROWTH REGULATION,2011,63(1):63-72
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Kranner, Ilse]'s Articles
[Chen, Hongying]'s Articles
[Pritchard, Hugh W.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Kranner, Ilse]‘s Articles
[Chen, Hongying]‘s Articles
[Pritchard, Hugh W.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 201204090009.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace