Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi

doi:10.1007/s11120-017-0389-4

	Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi
	Huang, Wei1,2 ; Yang, Ying-Jie 1; Zhang, Jiao-Lin 2; Hu, Hong1 ; Zhang, Shi-Bao1
	2017-06-01
发表期刊	PHOTOSYNTHESIS RESEARCH
卷号	132 页码:293-303
摘要	Our previous studies indicated that high light induced significant photoinhibition of photosystem I (PSI) in the shade-establishing tree species Psychotria henryi. However, the underlying mechanism has not been fully clarified. In the present study, in order to investigate the mechanism of PSI photoinhibition in P. henryi, we treated detached leaves with constant high light in the presence of methyl viologen (MV) or a soluble alpha-tocopherol analog, 2,2,5,7,8-pentamethyl-6-chromanol (PMC). We found that MV significantly depressed photochemical quantum yields in PSI and PSII when compared to PMC. On condition that no PSI photoinhibition happened, although cyclic electron flow (CEF) was abolished in the MV-treated samples, P700 oxidation ratio was maintain at higher levels than the PMC-treated samples. In the presence of PMC, PSI photoinhibition little changed but PSII photoinhibition was significantly alleviated. Importantly, PSI photoinhibition was largely accelerated in the presence of MV, which stimulates the production of superoxide and subsequently other reactive oxygen species at the chloroplast stroma by accepting electrons from PSI. Furthermore, MV largely aggravated PSII photoinhibition when compared to control. These results suggest that high P700 oxidation ratio cannot prevent PSI photoinhibition in P. henryi. Furthermore, the superoxide produced in the chloroplast stroma is critical for PSI photoinhibition in the higher plant P. henryi, which is opposite to the mechanism underlying PSI photoinhibition in Arabidopsis thaliana and spinach. These findings highlight a new mechanism of PSI photoinhibition in higher plants.
关键词	Chlorophyll Fluorescence Methyl Viologen P700 Oxidation Photosystem i Photoinhibition Superoxide
DOI	10.1007/s11120-017-0389-4
收录类别	SCI
语种	英语
WOS记录号	WOS:000402083700008
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/51358
专题	资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Forest Ecol, Mengla 666303, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Huang, Wei,Yang, Ying-Jie,Zhang, Jiao-Lin,et al. Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi[J]. PHOTOSYNTHESIS RESEARCH,2017,132:293-303.
APA	Huang, Wei,Yang, Ying-Jie,Zhang, Jiao-Lin,Hu, Hong,&Zhang, Shi-Bao.(2017).Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi.PHOTOSYNTHESIS RESEARCH,132,293-303.
MLA	Huang, Wei,et al."Superoxide generated in the chloroplast stroma causes photoinhibition of photosystem I in the shade-establishing tree species Psychotria henryi".PHOTOSYNTHESIS RESEARCH 132(2017):293-303.

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