Quantitative proteomics analysis of Caenorhabditis elegans upon germ cell loss
Pu, Yuan-Zhu1,2; Wan, Qin-Li1,2; Ding, Ai-Jun1,2; Luo, Huai-Rong1,3; Wu, Gui-Sheng1,3
AbstractThe abrogation of the germ cells in Caenorhabditis elegans (C. elegans) by either genetic means or cell ablation results in about 60% increase of longevity. Upon the inhibition of germline stem cell proliferation, certain signaling molecules inhibit the target of rapamycin (TOR), activate the transcription factors including DAF-16, DAP-12, and PHA-4, leading to altered fatty acid I ipolysis, autophagy, stress resistance, and the extended lifespan. But the exact cascades and interactions of those signaling pathways are still obscure. To understand how the reproductive system affects aging at the protein level, we determined the protein expression profile of the long-lived temperature-sensitive mutant glp-1(e2141) and wild-type N2 using isobaric tags for relative and absolute quantitation (IRAQ) technology. Our results showed that the abundance of proteins relevant to transcription, RNA processing, translation, protein folding, and proteolytic process were decreased, while collagen proteins and proteins involved in detoxification and innate immune responses were increased in C elegans glp-1 mutant, these alterations of protein abundance might attenuate protein metabolism and enhance immune response and stress resistance, and finally contribute to germline-mediated longevity.
KeywordCaenorhabditis Elegans Proteomics Aging Notch Receptor Glp-1 Reproduction
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000395609700009
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, State Key Lab Phytochem & Plant Resources West Ch, 134 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, PR, Peoples R China
3.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Key Lab Aging & Regenerat Med, Luzhou, Sichuan 646000, Peoples R China
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GB/T 7714
Pu, Yuan-Zhu,Wan, Qin-Li,Ding, Ai-Jun,et al. Quantitative proteomics analysis of Caenorhabditis elegans upon germ cell loss[J]. JOURNAL OF PROTEOMICS,2017,156(1):85-93.
APA Pu, Yuan-Zhu,Wan, Qin-Li,Ding, Ai-Jun,Luo, Huai-Rong,&Wu, Gui-Sheng.(2017).Quantitative proteomics analysis of Caenorhabditis elegans upon germ cell loss.JOURNAL OF PROTEOMICS,156(1),85-93.
MLA Pu, Yuan-Zhu,et al."Quantitative proteomics analysis of Caenorhabditis elegans upon germ cell loss".JOURNAL OF PROTEOMICS 156.1(2017):85-93.
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