Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans
Wan, Qin-Li1,2,3; Shi, Xiaohuo1,3; Liu, Jiangxin1,3; Ding, Ai-Jun1,2,3; Pu, Yuan-Zhu1,2,3; Li, Zhigang4; Wu, Gui-Sheng1,3,5; Luo, Huai-Rong1,3,5
2017-02-01
Source PublicationAGING-US
Volume9Issue:2Pages:447-474
AbstractIn Caenorhabditis elegans (C. elegans), ablation of germline stem cells (GSCs) leads to infertility, which extends lifespan. It has been reported that aging and reproduction are both inextricably associated with metabolism. However, few studies have investigated the roles of polar small molecules metabolism in regulating longevity by reproduction. In this work, we combined the nuclear magnetic resonance (NMR) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) to profile the water-soluble metabolome in C. elegans. Comparing the metabolic fingerprint between two physiological ages among different mutants, our results demonstrate that aging is characterized by metabolome remodeling and metabolic decline. In addition, by analyzing the metabolic profiles of long-lived germline-less glp-1 mutants, we discovered that glp-1 mutants regulate the levels of many age-variant metabolites to attenuate aging, including elevated concentrations of the pyrimidine and purine metabolism intermediates and decreased concentrations of the citric acid cycle intermediates. Interestingly, by analyzing the metabolome of daf-16;glp-1 double mutants, our results revealed that some metabolic exchange contributing to germline-mediated longevity was mediated by transcription factor FOXO/DAF-16, including pyrimidine metabolism and the TCA cycle. Based on a comprehensive metabolic analysis, we provide novel insight into the relationship between longevity and metabolism regulated by germline signals in C. elegans.
KeywordCaenorhabditis Elegans Aging Reproduction Metabolome Uplc-ms And Nmr
DOI10.18632/aging.101170
Indexed BySCI
Language英语
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000396892500013
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/50999
Collection植物化学与西部植物资源持续利用国家重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
4.Shineway Pharmaceut Co Ltd, Sanhe 065201, Hebei, Peoples R China
5.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Key Lab Aging & Regenerat Med, Luzhou 646000, Sichuan, Peoples R China
Recommended Citation
GB/T 7714
Wan, Qin-Li,Shi, Xiaohuo,Liu, Jiangxin,et al. Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans[J]. AGING-US,2017,9(2):447-474.
APA Wan, Qin-Li.,Shi, Xiaohuo.,Liu, Jiangxin.,Ding, Ai-Jun.,Pu, Yuan-Zhu.,...&Luo, Huai-Rong.(2017).Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans.AGING-US,9(2),447-474.
MLA Wan, Qin-Li,et al."Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans".AGING-US 9.2(2017):447-474.
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