| Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans | |
Wan, Qin-Li1,2,3; Shi, Xiaohuo1,3; Liu, Jiangxin1,3; Ding, Ai-Jun1,2,3; Pu, Yuan-Zhu1,2,3; Li, Zhigang4; Wu, Gui-Sheng1,3,5 ; Luo, Huai-Rong1,3,5
| |
| 2017-02-01 | |
| 发表期刊 | AGING-US
 |
| 卷号 | 9期号:2页码:447-474 |
| 摘要 | In Caenorhabditis elegans (C. elegans), ablation of germline stem cells (GSCs) leads to infertility, which extends lifespan. It has been reported that aging and reproduction are both inextricably associated with metabolism. However, few studies have investigated the roles of polar small molecules metabolism in regulating longevity by reproduction. In this work, we combined the nuclear magnetic resonance (NMR) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) to profile the water-soluble metabolome in C. elegans. Comparing the metabolic fingerprint between two physiological ages among different mutants, our results demonstrate that aging is characterized by metabolome remodeling and metabolic decline. In addition, by analyzing the metabolic profiles of long-lived germline-less glp-1 mutants, we discovered that glp-1 mutants regulate the levels of many age-variant metabolites to attenuate aging, including elevated concentrations of the pyrimidine and purine metabolism intermediates and decreased concentrations of the citric acid cycle intermediates. Interestingly, by analyzing the metabolome of daf-16;glp-1 double mutants, our results revealed that some metabolic exchange contributing to germline-mediated longevity was mediated by transcription factor FOXO/DAF-16, including pyrimidine metabolism and the TCA cycle. Based on a comprehensive metabolic analysis, we provide novel insight into the relationship between longevity and metabolism regulated by germline signals in C. elegans. |
| 关键词 | Caenorhabditis Elegans Aging Reproduction Metabolome Uplc-ms And Nmr |
| DOI | 10.18632/aging.101170 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000396892500013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/50999 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China 4.Shineway Pharmaceut Co Ltd, Sanhe 065201, Hebei, Peoples R China 5.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Key Lab Aging & Regenerat Med, Luzhou 646000, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Qin-Li,Shi, Xiaohuo,Liu, Jiangxin,et al. Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans[J]. AGING-US,2017,9(2):447-474. |
| APA | Wan, Qin-Li.,Shi, Xiaohuo.,Liu, Jiangxin.,Ding, Ai-Jun.,Pu, Yuan-Zhu.,...&Luo, Huai-Rong.(2017).Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans.AGING-US,9(2),447-474. |
| MLA | Wan, Qin-Li,et al."Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans".AGING-US 9.2(2017):447-474. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| aging-09-447.pdf（4040KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论