| microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways | |
Wei, Ran1,2; Yang, Qin1; Han, Bing1,3; Li, Yan1 ; Yao, Kun1; Yang, Xiuyu1; Chen, Zexi1,3; Yang, Shanshan1; Zhou, Jiaqi1; Li, Meizhang1; Yu, Haijing1; Yu, Min1; Cui, Qinghua1,2
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| 2017 | |
| 发表期刊 | ONCOTARGET
 |
| 卷号 | 8期号:10页码:16633-16641 |
| 摘要 | MicroRNA-375 is involved in many types of alimentary system cancers. Our previous studies showed that microRNA-375 was significantly down-regulated in carcinoma tissues compared with para-carcinoma tissues, which strongly indicates that microRNA-375 might suppress the occurrence and development of colorectal cancer. However, the mechanism underlying the microRNA-375 regulation in colorectal cancer remains unclear. In this study, we first sorted out jak2, map3k8 and atg7 as microRNA-375 targeted genes from multiple databases, and found that jak2, map3k8 and their downstream genes stat3 and erk were up-regulated in carcinoma tissues. Secondly, we over-expressed microRNA-375 in colorectal cancer cell lines (HCT116, Caco2 and HT29). Our results showed that in microRNA-375 over-expressing cells, JAK2/STAT3 and MAP3K8/ERK proteins were down-regulated, cell proliferation was inhibited, cell migration rate did not change. There was no significant difference on ATG7 expression between the control group and microRNA-375 over-expressing HT29/ Caco2 cells, whereas microRNA-375 down-regulated ATG7 specifically in HCT116 cells. Finally, we demonstrated that expressing microRNA-375 suppressed tumor formation in nude mice. In conclusion, microRNA-375 might function as a tumor-repressive gene to inhibit cell proliferation, mainly through targeting both JAK2/STAT3 and MAP3K8/ERK signaling pathways in colorectal cancer. These findings suggest miR-375 as a promising diagnostic marker and a therapeutic drug for colorectal cancer. |
| 关键词 | Microrna-375 Colorectal Cancer Jak2/stat3 Pathway Map3k8/erk Pathway Atg7 |
| DOI | 10.18632/oncotarget.15114 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000396024600045 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/46045 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China 2.Yunnan Univ, Key Lab Tumor Mol Biol Yunnan Prov, Kunming 650091, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Ran,Yang, Qin,Han, Bing,et al. microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways[J]. ONCOTARGET,2017,8(10):16633-16641. |
| APA | Wei, Ran.,Yang, Qin.,Han, Bing.,Li, Yan.,Yao, Kun.,...&Cui, Qinghua.(2017).microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways.ONCOTARGET,8(10),16633-16641. |
| MLA | Wei, Ran,et al."microRNA-375 inhibits colorectal cancer cells proliferation by downregulating JAK2/STAT3 and MAP3K8/ERK signaling pathways".ONCOTARGET 8.10(2017):16633-16641. |
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| 15114-224062-2-PB.pd（12068KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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