Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder
Takagi, Hiroki1; Sugawara, Shintaro1; Saito, Tomoka1; Tasaki, Haruka1; Lu Yuanxue2; Guan Kaiyun2; Han, Dong-Sheng1; Godo, Toshinari3; Nakano, Masaru1
Corresponding Authormnakano@agr.niigata-u.ac.jp
2011-04-01
Source PublicationPLANT BIOTECHNOLOGY REPORTS
ISSN1863-5466
Volume5Issue:2Pages:187-195
AbstractThe gesneriaceous perennial plant Titanotrichum oldhamii has beautiful foliage and attractive bright yellow flowers. However, breeding of T. oldhamii by conventional sexual hybridization may be difficult because sexual reproduction of this species is very rare. In the present study, plant regeneration systems via both direct and indirect formation of adventitious shoots from leaf explants were established as the first step toward breeding T. oldhamii by using biotechnological techniques. Adventitious shoots were formed efficiently on medium containing 0.1 mg l(-1) benzyladenine. Histological observation showed that shoot formation on this medium occurred directly from leaf epidermal cells without callus formation. On the other hand, leaf explants formed calluses on medium containing 0.1 mg l(-1) 2,4-dichlorophenoxyacetic acid. The calluses could be maintained by monthly subculturing to fresh medium of the same composition. When the calluses were transferred to plant growth regulator-free medium, they formed adventitious shoots. Directly and indirectly formed shoots rooted well on medium containing 0.1 mg l(-1) indole-3-butyric acid. Plantlets thus obtained were successfully acclimatized and grew vigorously in the greenhouse. Flow cytometry analysis indicated that no variation in the ploidy level was observed in plants regenerated via direct shoot formation, whereas chromosome doubling occurred in several plants regenerated via indirect shoot formation. Regenerated plants with the same ploidy level as the mother plants showed almost the same phenotype as the mother plants, whereas chromosome-doubled plants showed apparent morphological alterations: they had small and crispate flowers, and round and deep green leaves.
KeywordFlow Cytometry Gesneriaceous Plant Histological Observation Plant Regeneration Ploidy Level Somaclonal Variation
Subject AreaBiotechnology & Applied Microbiology ; Plant Sciences
DOI10.1007/s11816-011-0172-5
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Plant Sciences
WOS SubjectBiotechnology & Applied Microbiology ; Plant Sciences
WOS IDWOS:000289441700009
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/3990
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Niigata Univ, Fac Agr, Niigata 9502181, Japan
2.Chinese Acad Sci, Kunming Bot Garden, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
3.Bot Gardens Toyama, Fuchu, Tokyo 9392713, Japan
Recommended Citation
GB/T 7714
Takagi, Hiroki,Sugawara, Shintaro,Saito, Tomoka,et al. Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder[J]. PLANT BIOTECHNOLOGY REPORTS,2011,5(2):187-195.
APA Takagi, Hiroki.,Sugawara, Shintaro.,Saito, Tomoka.,Tasaki, Haruka.,Lu Yuanxue.,...&Nakano, Masaru.(2011).Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder.PLANT BIOTECHNOLOGY REPORTS,5(2),187-195.
MLA Takagi, Hiroki,et al."Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder".PLANT BIOTECHNOLOGY REPORTS 5.2(2011):187-195.
Files in This Item: Download All
File Name/Size DocType Version Access License
201203160040.pdf(589KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Takagi, Hiroki]'s Articles
[Sugawara, Shintaro]'s Articles
[Saito, Tomoka]'s Articles
Baidu academic
Similar articles in Baidu academic
[Takagi, Hiroki]'s Articles
[Sugawara, Shintaro]'s Articles
[Saito, Tomoka]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Takagi, Hiroki]'s Articles
[Sugawara, Shintaro]'s Articles
[Saito, Tomoka]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 201203160040.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.