Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder
Takagi, Hiroki1; Sugawara, Shintaro1; Saito, Tomoka1; Tasaki, Haruka1; Lu Yuanxue2; Guan Kaiyun2; Han, Dong-Sheng1; Godo, Toshinari3; Nakano, Masaru1
2011-04-01
发表期刊PLANT BIOTECHNOLOGY REPORTS
ISSN1863-5466
卷号5期号:2页码:187-195
摘要The gesneriaceous perennial plant Titanotrichum oldhamii has beautiful foliage and attractive bright yellow flowers. However, breeding of T. oldhamii by conventional sexual hybridization may be difficult because sexual reproduction of this species is very rare. In the present study, plant regeneration systems via both direct and indirect formation of adventitious shoots from leaf explants were established as the first step toward breeding T. oldhamii by using biotechnological techniques. Adventitious shoots were formed efficiently on medium containing 0.1 mg l(-1) benzyladenine. Histological observation showed that shoot formation on this medium occurred directly from leaf epidermal cells without callus formation. On the other hand, leaf explants formed calluses on medium containing 0.1 mg l(-1) 2,4-dichlorophenoxyacetic acid. The calluses could be maintained by monthly subculturing to fresh medium of the same composition. When the calluses were transferred to plant growth regulator-free medium, they formed adventitious shoots. Directly and indirectly formed shoots rooted well on medium containing 0.1 mg l(-1) indole-3-butyric acid. Plantlets thus obtained were successfully acclimatized and grew vigorously in the greenhouse. Flow cytometry analysis indicated that no variation in the ploidy level was observed in plants regenerated via direct shoot formation, whereas chromosome doubling occurred in several plants regenerated via indirect shoot formation. Regenerated plants with the same ploidy level as the mother plants showed almost the same phenotype as the mother plants, whereas chromosome-doubled plants showed apparent morphological alterations: they had small and crispate flowers, and round and deep green leaves.
关键词Flow Cytometry Gesneriaceous Plant Histological Observation Plant Regeneration Ploidy Level Somaclonal Variation
学科领域Biotechnology & Applied Microbiology ; Plant Sciences
DOI10.1007/s11816-011-0172-5
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Plant Sciences
WOS类目Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号WOS:000289441700009
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被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3990
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Niigata Univ, Fac Agr, Niigata 9502181, Japan
2.Chinese Acad Sci, Kunming Bot Garden, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
3.Bot Gardens Toyama, Fuchu, Tokyo 9392713, Japan
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Takagi, Hiroki,Sugawara, Shintaro,Saito, Tomoka,et al. Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder[J]. PLANT BIOTECHNOLOGY REPORTS,2011,5(2):187-195.
APA Takagi, Hiroki.,Sugawara, Shintaro.,Saito, Tomoka.,Tasaki, Haruka.,Lu Yuanxue.,...&Nakano, Masaru.(2011).Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder.PLANT BIOTECHNOLOGY REPORTS,5(2),187-195.
MLA Takagi, Hiroki,et al."Plant regeneration via direct and indirect adventitious shoot formation and chromosome-doubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder".PLANT BIOTECHNOLOGY REPORTS 5.2(2011):187-195.
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