DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)
Ren, He1; Lu, Lu1,2; Wang, Hong1,2; Li, De-Zhu1,2
Corresponding Authorwanghong@mail.kib.ac.cn
2011-09-01
Source PublicationJOURNAL OF SYSTEMATICS AND EVOLUTION
ISSN1674-4918
Volume49Issue:5Pages:411-424
AbstractFour DNA barcoding loci, chloroplast loci rbcL, matK, trnH-psbA, and nuclear locus internal transcribed spacer (ITS), were tested for the accurate discrimination of the Chinese species of Gaultheria by using intraspecific and interspecific pairwise P-distance, Wilcoxon signed rank test, and tree-based analyses. This study included 186 individuals from 89 populations representing 30 species. For all individuals, single locus markers showed high levels of sequencing universality but were ineffective for species resolvability. Polymerase chain reaction amplification and sequencing were successful for all four loci. Both ITS and matK showed significantly higher levels of interspecific species delimitation than rbcL and trnH-psbA. A combination of matK and ITS was the most efficient DNA barcode among all studied regions, however, they do not represent an appropriate candidate barcode for Chinese Gaultheria, by which only 11 out of 30 species can be separated. Loci rbcL, matK, and trnH-psbA, which were recently proposed as universal plant barcodes, have a very poor capacity for species separation for Chinese Gaultheria. DNA barcodes may be reliable tools to identify the evolutionary units of this group, so further studies are needed to develop more efficient DNA barcodes for Gaultheria and other genera with complicated evolutionary histories.
KeywordDna Barcoding Ericaceae Gaultheria Species Discrimination Taxonomy Vaccinioideae
Subject AreaPlant Sciences
DOI10.1111/j.1759-6831.2011.00152.x
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000295322200005
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/3976
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Chinese Acad Sci, Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Peoples R China
Recommended Citation
GB/T 7714
Ren, He,Lu, Lu,Wang, Hong,et al. DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(5):411-424.
APA Ren, He,Lu, Lu,Wang, Hong,&Li, De-Zhu.(2011).DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae).JOURNAL OF SYSTEMATICS AND EVOLUTION,49(5),411-424.
MLA Ren, He,et al."DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.5(2011):411-424.
Files in This Item: Download All
File Name/Size DocType Version Access License
201203160033.pdf(186KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ren, He]'s Articles
[Lu, Lu]'s Articles
[Wang, Hong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ren, He]'s Articles
[Lu, Lu]'s Articles
[Wang, Hong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ren, He]'s Articles
[Lu, Lu]'s Articles
[Wang, Hong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 201203160033.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.