DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)
Ren, He1; Lu, Lu1,2; Wang, Hong1,2; Li, De-Zhu1,2
Corresponding Authorwanghong@mail.kib.ac.cn
AbstractFour DNA barcoding loci, chloroplast loci rbcL, matK, trnH-psbA, and nuclear locus internal transcribed spacer (ITS), were tested for the accurate discrimination of the Chinese species of Gaultheria by using intraspecific and interspecific pairwise P-distance, Wilcoxon signed rank test, and tree-based analyses. This study included 186 individuals from 89 populations representing 30 species. For all individuals, single locus markers showed high levels of sequencing universality but were ineffective for species resolvability. Polymerase chain reaction amplification and sequencing were successful for all four loci. Both ITS and matK showed significantly higher levels of interspecific species delimitation than rbcL and trnH-psbA. A combination of matK and ITS was the most efficient DNA barcode among all studied regions, however, they do not represent an appropriate candidate barcode for Chinese Gaultheria, by which only 11 out of 30 species can be separated. Loci rbcL, matK, and trnH-psbA, which were recently proposed as universal plant barcodes, have a very poor capacity for species separation for Chinese Gaultheria. DNA barcodes may be reliable tools to identify the evolutionary units of this group, so further studies are needed to develop more efficient DNA barcodes for Gaultheria and other genera with complicated evolutionary histories.
KeywordDna Barcoding Ericaceae Gaultheria Species Discrimination Taxonomy Vaccinioideae
Subject AreaPlant Sciences
Indexed BySCI
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000295322200005
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Peoples R China
Recommended Citation
GB/T 7714
Ren, He,Lu, Lu,Wang, Hong,et al. DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(5):411-424.
APA Ren, He,Lu, Lu,Wang, Hong,&Li, De-Zhu.(2011).DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae).JOURNAL OF SYSTEMATICS AND EVOLUTION,49(5),411-424.
MLA Ren, He,et al."DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.5(2011):411-424.
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