DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)
Ren, He1; Lu, Lu1,2; Wang, Hong1,2; Li, De-Zhu1,2
2011-09-01
发表期刊JOURNAL OF SYSTEMATICS AND EVOLUTION
ISSN1674-4918
卷号49期号:5页码:411-424
摘要Four DNA barcoding loci, chloroplast loci rbcL, matK, trnH-psbA, and nuclear locus internal transcribed spacer (ITS), were tested for the accurate discrimination of the Chinese species of Gaultheria by using intraspecific and interspecific pairwise P-distance, Wilcoxon signed rank test, and tree-based analyses. This study included 186 individuals from 89 populations representing 30 species. For all individuals, single locus markers showed high levels of sequencing universality but were ineffective for species resolvability. Polymerase chain reaction amplification and sequencing were successful for all four loci. Both ITS and matK showed significantly higher levels of interspecific species delimitation than rbcL and trnH-psbA. A combination of matK and ITS was the most efficient DNA barcode among all studied regions, however, they do not represent an appropriate candidate barcode for Chinese Gaultheria, by which only 11 out of 30 species can be separated. Loci rbcL, matK, and trnH-psbA, which were recently proposed as universal plant barcodes, have a very poor capacity for species separation for Chinese Gaultheria. DNA barcodes may be reliable tools to identify the evolutionary units of this group, so further studies are needed to develop more efficient DNA barcodes for Gaultheria and other genera with complicated evolutionary histories.
关键词Dna Barcoding Ericaceae Gaultheria Species Discrimination Taxonomy Vaccinioideae
资助信息Chinese Academy of Sciences[2009-LSF-GBOWS-01]; Western Light Talent Culture Project[2010312D11034]
学科领域Plant Sciences
DOI10.1111/j.1759-6831.2011.00152.x
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000295322200005
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3976
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Peoples R China
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Ren, He,Lu, Lu,Wang, Hong,et al. DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(5):411-424.
APA Ren, He,Lu, Lu,Wang, Hong,&Li, De-Zhu.(2011).DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae).JOURNAL OF SYSTEMATICS AND EVOLUTION,49(5),411-424.
MLA Ren, He,et al."DNA barcoding of Gaultheria L. in China (Ericaceae: Vaccinioideae)".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.5(2011):411-424.
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