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题名: A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China
作者: Ma, Hong ; Wang, Lan ; Wan, Youming ; Li, Hongzhe ; Li, Zhenghong ; Liu, Xiuxian ; Liang, Ning ; Li, Wenjuan
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
关键词: genetic diversity ; Luculia yunnanensis ; microsatellite marker ; polymorphism ; population structure ; Rubiaceae
英文摘要: The genus Luculia Sweet contains about five species of small trees or shrubs and is a member of the family Rubiaceae (tribe Cinchoneae). Luculia yunnanensis is an endangered ornamental shrub endemic to southwest China. Only two natural populations of L. yunnanensis exist in the wild according to our field investigation. It can be inferred that L. yunnanensis is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-sterptavidin capture method, 24 primer sets were identified in two wild populations. Of these primers, 11 displayed polymorphisms and 13 were monomorphic. The number of alleles per locus ranged from two to four, values for observed and expected heterozygosities ranged from 0.000 to 0.833 and from 0.431 to 0.771, with averages of 0.389 and 0.614, respectively. These markers will be useful for further investigation of conservation of resources, selecting parental types in cross-breeding, evolution of this species at the molecular level and related research in Luculia species.
出版日期: 2012
卷号: 13, 期号:1, 页码:534-539
语种: 英语
收录类别: sci
学科分类: Chemistry, Multidisciplinary
ISSN号: 1661-6596
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/3972
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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Ma, Hong; Wang, Lan; Wan, Youming; Li, Hongzhe; Li, Zhenghong; Liu, Xiuxian; Liang, Ning; Li, Wenjuan.A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China,INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(1):534-539
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