A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China
Ma, Hong ; Wang, Lan ; Wan, Youming ; Li, Hongzhe ; Li, Zhenghong ; Liu, Xiuxian ; Liang, Ning ; Li, Wenjuan
2012
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
卷号13期号:1页码:534-539
摘要The genus Luculia Sweet contains about five species of small trees or shrubs and is a member of the family Rubiaceae (tribe Cinchoneae). Luculia yunnanensis is an endangered ornamental shrub endemic to southwest China. Only two natural populations of L. yunnanensis exist in the wild according to our field investigation. It can be inferred that L. yunnanensis is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-sterptavidin capture method, 24 primer sets were identified in two wild populations. Of these primers, 11 displayed polymorphisms and 13 were monomorphic. The number of alleles per locus ranged from two to four, values for observed and expected heterozygosities ranged from 0.000 to 0.833 and from 0.431 to 0.771, with averages of 0.389 and 0.614, respectively. These markers will be useful for further investigation of conservation of resources, selecting parental types in cross-breeding, evolution of this species at the molecular level and related research in Luculia species.
关键词Genetic Diversity Luculia Yunnanensis Microsatellite Marker Polymorphism Population Structure Rubiaceae
资助信息Special Fund of Basic Research of Central Nonprofit Research Institutes[Riricaf 201003M]
学科领域Chemistry, Multidisciplinary
收录类别sci
语种英语
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3972
专题中国科学院东亚植物多样性与生物地理学重点实验室
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Ma, Hong,Wang, Lan,Wan, Youming,et al. A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(1):534-539.
APA Ma, Hong.,Wang, Lan.,Wan, Youming.,Li, Hongzhe.,Li, Zhenghong.,...&Li, Wenjuan.(2012).A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(1),534-539.
MLA Ma, Hong,et al."A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.1(2012):534-539.
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