| Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb | |
Li, Xiang1,2,3; Song, Kexian1,3,4; Yang, Junbo1; Yi, Tingshuang1,3
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| 通讯作者 | lixiangjohnlily@126.com ; songkexian555@yahoo.com.cn ; jbyang@mail.kib.ac.cn ; tingshuangyi@mail.kib.ac.cn |
| 2011-10-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| ISSN | 1661-6596 |
| 卷号 | 12期号:10页码:7265-7270 |
| 摘要 | Erigeron breviscapus (Vant.) Hand.-Mazz. (Asteraceae) is a species endemic to southwestern China and an important traditional Chinese herb for cardiovascular and cerebral vessel diseases. Applying a modified biotin-streptavidin capture method, 11 microsatellite loci were discovered. Polymorphism of each locus was assessed in 24 individuals collected from five wild populations. The number of alleles per locus ranged from 2 to 7, with an average of 4.273. The observed (HO) and expected (HE) heterozygosities varied from 0.250 to 0.958 and from 0.337 to 0.786, respectively. Over half of these loci were successfully amplified in two congeneric species. The developed microsatellite markers will be useful for future population genetics and conservation studies, as well as accurate identification of different varieties. |
| 关键词 | Erigeron Breviscapus Microsatellite Markers Polymorphism Population Genetics |
| 学科领域 | Chemistry, Multidisciplinary |
| DOI | 10.3390/ijms12107265 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296569600055 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/3944 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650201, Peoples R China 4.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiang,Song, Kexian,Yang, Junbo,et al. Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(10):7265-7270. |
| APA | Li, Xiang,Song, Kexian,Yang, Junbo,&Yi, Tingshuang.(2011).Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(10),7265-7270. |
| MLA | Li, Xiang,et al."Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.10(2011):7265-7270. |
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