Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb
Li, Xiang1,2,3; Song, Kexian1,3,4; Yang, Junbo1; Yi, Tingshuang1,3
2011-10-01
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
卷号12期号:10页码:7265-7270
摘要Erigeron breviscapus (Vant.) Hand.-Mazz. (Asteraceae) is a species endemic to southwestern China and an important traditional Chinese herb for cardiovascular and cerebral vessel diseases. Applying a modified biotin-streptavidin capture method, 11 microsatellite loci were discovered. Polymorphism of each locus was assessed in 24 individuals collected from five wild populations. The number of alleles per locus ranged from 2 to 7, with an average of 4.273. The observed (HO) and expected (HE) heterozygosities varied from 0.250 to 0.958 and from 0.337 to 0.786, respectively. Over half of these loci were successfully amplified in two congeneric species. The developed microsatellite markers will be useful for future population genetics and conservation studies, as well as accurate identification of different varieties.
关键词Erigeron Breviscapus Microsatellite Markers Polymorphism Population Genetics
资助信息State Key Laboratory of Phytochemistry and Plant Resources in West China; Germplasm Bank of Wild Species (Kunming Insititute of Botany, Chinese Academy of Sciences); CAS of Kunming Institute[540806321211]
学科领域Chemistry, Multidisciplinary
DOI10.3390/ijms12107265
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000296569600055
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3944
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650201, Peoples R China
4.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China
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Li, Xiang,Song, Kexian,Yang, Junbo,et al. Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(10):7265-7270.
APA Li, Xiang,Song, Kexian,Yang, Junbo,&Yi, Tingshuang.(2011).Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(10),7265-7270.
MLA Li, Xiang,et al."Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.10(2011):7265-7270.
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