Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)

doi:10.5897/AJB11.1877

	Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)
	Li, Rong; Dao, Zhiling
通讯作者	daozhl@mail.kib.ac.cn
	2011-11-09
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN	1684-5315
卷号	10 期号:70 页码:15805-15807
摘要	Deoxyribonucleic acid (DNA) barcoding is a novel technology that uses a standard DNA sequence to facilitate species identification. Species identification is necessary for the authentication of traditional plant based medicines. Although a consensus has not been agreed regarding which DNA sequences can be used as the best plant barcode, the internal transcribed spacer (ITS) region of nuclear ribosomal DNA has been tested and applied extensively in recent years. In this study, we used the ITS region to test its possibility to serve as barcodes for Meconopsis species which are widely used in traditional Tibetan medicines that are used throughout the Himalayas. We sequenced 14 accessions representing four Meconopsis species. The result shows ITS sequences were found to be unique for each species, with the interspecies percentages of nucleotide differences averaging 5.1% and ranging from 0.2 to 9.6%. In contrast, the intraspecies percentages of nucleotide differences among the Meconopsis species studied ranged from 0 to 1.9%. Our results indicate that the ITS region can be used as a barcode to distinguish Meconopsis species.
关键词	Dna Barcoding Species Identification Meconopsis Its Region
学科领域	Biotechnology & Applied Microbiology
DOI	10.5897/AJB11.1877
收录类别	SCI
语种	英语
WOS记录号	WOS:000298555900021
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/3922
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Rong Li, Zhiling Dao. 2011. Identification of Meconopsis species by a DNA barcode sequence: the nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA). African Journal of Biotechnology 10: 15805-15807.
APA	Li, Rong,&Dao, Zhiling.(2011).Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA).AFRICAN JOURNAL OF BIOTECHNOLOGY,10(70),15805-15807.
MLA	Li, Rong,et al."Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.70(2011):15805-15807.

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