Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)
Li, Rong; Dao, Zhiling
2011-11-09
发表期刊AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN1684-5315
卷号10期号:70页码:15805-15807
摘要Deoxyribonucleic acid (DNA) barcoding is a novel technology that uses a standard DNA sequence to facilitate species identification. Species identification is necessary for the authentication of traditional plant based medicines. Although a consensus has not been agreed regarding which DNA sequences can be used as the best plant barcode, the internal transcribed spacer (ITS) region of nuclear ribosomal DNA has been tested and applied extensively in recent years. In this study, we used the ITS region to test its possibility to serve as barcodes for Meconopsis species which are widely used in traditional Tibetan medicines that are used throughout the Himalayas. We sequenced 14 accessions representing four Meconopsis species. The result shows ITS sequences were found to be unique for each species, with the interspecies percentages of nucleotide differences averaging 5.1% and ranging from 0.2 to 9.6%. In contrast, the intraspecies percentages of nucleotide differences among the Meconopsis species studied ranged from 0 to 1.9%. Our results indicate that the ITS region can be used as a barcode to distinguish Meconopsis species.
关键词Dna Barcoding Species Identification Meconopsis Its Region
资助信息Chinese Academy of Sciences[2010KIBA06]
学科领域Biotechnology & Applied Microbiology
DOI10.5897/AJB11.1877
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000298555900021
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3922
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
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Rong Li, Zhiling Dao. 2011. Identification of Meconopsis species by a DNA barcode sequence: the nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA). African Journal of Biotechnology 10: 15805-15807.
APA Li, Rong,&Dao, Zhiling.(2011).Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA).AFRICAN JOURNAL OF BIOTECHNOLOGY,10(70),15805-15807.
MLA Li, Rong,et al."Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.70(2011):15805-15807.
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