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题名: Identification of Meconopsis species by a DNA barcode sequence: The nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA)
作者: null(李嵘)1; Dao, Zhiling1
刊名: AFRICAN JOURNAL OF BIOTECHNOLOGY
关键词: DNA barcoding ; species identification ; Meconopsis ; ITS region
英文摘要: Deoxyribonucleic acid (DNA) barcoding is a novel technology that uses a standard DNA sequence to facilitate species identification. Species identification is necessary for the authentication of traditional plant based medicines. Although a consensus has not been agreed regarding which DNA sequences can be used as the best plant barcode, the internal transcribed spacer (ITS) region of nuclear ribosomal DNA has been tested and applied extensively in recent years. In this study, we used the ITS region to test its possibility to serve as barcodes for Meconopsis species which are widely used in traditional Tibetan medicines that are used throughout the Himalayas. We sequenced 14 accessions representing four Meconopsis species. The result shows ITS sequences were found to be unique for each species, with the interspecies percentages of nucleotide differences averaging 5.1% and ranging from 0.2 to 9.6%. In contrast, the intraspecies percentages of nucleotide differences among the Meconopsis species studied ranged from 0 to 1.9%. Our results indicate that the ITS region can be used as a barcode to distinguish Meconopsis species.
出版日期: 2011-11-09
卷号: 10, 期号:70, 页码:15805-15807
DOI标识: 10.5897/AJB11.1877
语种: 英语
收录类别: SCI
学科分类: Biotechnology & Applied Microbiology
ISSN号: 1684-5315
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/3922
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China

Recommended Citation:
Rong Li, Zhiling Dao. 2011. Identification of Meconopsis species by a DNA barcode sequence: the nuclear internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid (DNA). African Journal of Biotechnology 10: 15805-15807.
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