| Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae) | |
Chen, Shaotian1; Wu, Xiayu2; Ji, Yunheng1 ; Yang, Junbo3
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| 通讯作者 | chenst@kib.ac ; xiayu98wu@yahoo.com.cn ; jiyh@mail.kib.ac.cn ; jbyang@mail.kib.ac.cn |
| 2011-11-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| ISSN | 1661-6596 |
| 卷号 | 12期号:11页码:7818-7823 |
| 摘要 | Fourteen polymorphic microsatellite loci were isolated from the genomic DNA of Pistacia weinmannifolia, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) method, and screened on 12 individuals from each of two wild populations. The 14 polymorphic loci had an average of 4.1 alleles per locus varying from 1 to 9. The observed (H-o) and expected (He) heterozygosities across the two populations ranged from 0.000 to 0.933 and from 0.000 to 0.906, respectively. Tests for departure from Hardy-Weinberg equilibrium (HWE) and genotypic linkage disequilibrium (LD) were conducted for each of the two populations separately. It was found that no locus significantly deviated from HWE proportions and no significant LD was detected between loci (p < 0.001). In the test of cross-species utility, we successfully amplified nine (64.2%) of 14 loci in P. chinensis and four (28.6%) in P. mexicana. The relatively high level of polymorphism for these markers will facilitate further studies of gene flow, population structure and evolutionary history of P. weinmannifolia and its congeners. |
| 关键词 | Pistacia Weinmannifolia Microsatellites Anacardiaceae Genetic Structure Polymorphism |
| 学科领域 | Chemistry, Multidisciplinary |
| DOI | 10.3390/ijms12117818 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297696100037 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/3898 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China 2.Yunnan Normal Univ, Sch Life Sci, Kunming 650092, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650204, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Shaotian,Wu, Xiayu,Ji, Yunheng,et al. Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(11):7818-7823. |
| APA | Chen, Shaotian,Wu, Xiayu,Ji, Yunheng,&Yang, Junbo.(2011).Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(11),7818-7823. |
| MLA | Chen, Shaotian,et al."Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.11(2011):7818-7823. |
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