Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)
Chen, Shaotian1; Wu, Xiayu2; Ji, Yunheng1; Yang, Junbo3
2011-11-01
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
卷号12期号:11页码:7818-7823
摘要Fourteen polymorphic microsatellite loci were isolated from the genomic DNA of Pistacia weinmannifolia, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) method, and screened on 12 individuals from each of two wild populations. The 14 polymorphic loci had an average of 4.1 alleles per locus varying from 1 to 9. The observed (H-o) and expected (He) heterozygosities across the two populations ranged from 0.000 to 0.933 and from 0.000 to 0.906, respectively. Tests for departure from Hardy-Weinberg equilibrium (HWE) and genotypic linkage disequilibrium (LD) were conducted for each of the two populations separately. It was found that no locus significantly deviated from HWE proportions and no significant LD was detected between loci (p < 0.001). In the test of cross-species utility, we successfully amplified nine (64.2%) of 14 loci in P. chinensis and four (28.6%) in P. mexicana. The relatively high level of polymorphism for these markers will facilitate further studies of gene flow, population structure and evolutionary history of P. weinmannifolia and its congeners.
关键词Pistacia Weinmannifolia Microsatellites Anacardiaceae Genetic Structure Polymorphism
资助信息National Natural Science Foundation of China[30770137, 31070198]; Chinese Academy of Sciences[2009-LSF-GBOWS-01]; GBOWS
学科领域Chemistry, Multidisciplinary
DOI10.3390/ijms12117818
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000297696100037
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3898
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China
2.Yunnan Normal Univ, Sch Life Sci, Kunming 650092, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650204, Peoples R China
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Chen, Shaotian,Wu, Xiayu,Ji, Yunheng,et al. Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(11):7818-7823.
APA Chen, Shaotian,Wu, Xiayu,Ji, Yunheng,&Yang, Junbo.(2011).Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(11),7818-7823.
MLA Chen, Shaotian,et al."Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.11(2011):7818-7823.
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