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题名: Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)
作者: Chen, Shaotian1; Wu, Xiayu2; null(纪云恒)1; Yang, Junbo3
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
关键词: Pistacia weinmannifolia ; microsatellites ; Anacardiaceae ; genetic structure ; polymorphism
英文摘要: Fourteen polymorphic microsatellite loci were isolated from the genomic DNA of Pistacia weinmannifolia, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) method, and screened on 12 individuals from each of two wild populations. The 14 polymorphic loci had an average of 4.1 alleles per locus varying from 1 to 9. The observed (H-o) and expected (He) heterozygosities across the two populations ranged from 0.000 to 0.933 and from 0.000 to 0.906, respectively. Tests for departure from Hardy-Weinberg equilibrium (HWE) and genotypic linkage disequilibrium (LD) were conducted for each of the two populations separately. It was found that no locus significantly deviated from HWE proportions and no significant LD was detected between loci (p < 0.001). In the test of cross-species utility, we successfully amplified nine (64.2%) of 14 loci in P. chinensis and four (28.6%) in P. mexicana. The relatively high level of polymorphism for these markers will facilitate further studies of gene flow, population structure and evolutionary history of P. weinmannifolia and its congeners.
出版日期: 2011-11-01
卷号: 12, 期号:11, 页码:7818-7823
DOI标识: 10.3390/ijms12117818
语种: 英语
ISSN号: 1661-6596
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/3898
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China
2.Yunnan Normal Univ, Sch Life Sci, Kunming 650092, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650204, Peoples R China

Recommended Citation:
Chen, Shaotian; Wu, Xiayu; Ji, Yunheng; Yang, Junbo.Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae),INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(11):7818-7823
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