7、最终结果表明运用染色体基因组原位杂交（GISH）的方法和技术已探明了滇山茶六倍体三个主要亲本种基因组成分和其他近缘种渗入部分基因片段成分。可以说我们已获得了证明滇山茶六倍体起源的实验结果和理论。; Camellia reticulata， named Yunnan Camellia, is one of the ten well-known flower in China, is also famouse in the world as an important flowering ornamental species. Clarifying the orgin and evolution of C. reticulata will has some important theoretical significance, and has important value for the rational exploitation and utilization of C. reticulata germplasm resouces. In order to reveal the origin of the C. reticulata polyploid complex, FISH was used. Meanwhile, AFLP, rDNA waxy sequence and four cpDNA sequences were employed to clearify the relationships between C. reticulata polyploid complex and its related species. The main results are as follows:
1, Phylogeny of C. reticulata polyploid complex and its related species were studied by maximum parsimony analysis of nuclear DNA waxy sequence and 4 chloroplast DNA sequence (trnL-F, trnT-L, trnS-G and rpL16 intron), respectively and unitedly. The results showed that C. pitardii, C. saluenensis, C. semiserrata, C. polyodonta and C. mariei var. lapidea were close related species of C. reticulata polyploid complex.
2, Amplified fragment length polymorphisms (AFLP) was used to analyze the relationship between C. reticulata polyploid complex and its related species. The result was the same as that of gene sequence analysis.
3, Strong signals were observed on the whold chromosomes when the gDNA (genomic DNA) of C. reticulata was as its own probe. All of the signals were located on every chromosome.
4, Signals were observed at the end of chromosomes when tetraploid C. reticulata was the target, diploid C. reticulata, C. saluenensis, C. semiserata, C. polyodonta and C. japonica was the probe respectively. With diploid C. reticulata and C. pitardii as probe respectively, strong signals were observed at half of chromosomes in tetraploid C. reticulata. The results supported that tetraploid C. reticulata is an allotetraploid species, and diploid C. reticulata and C. pitardii are the diploid progenitors of tetraploid C. reticulata.
5, Signals were observed mainly at the end of chromosomes with hexaploid C. reticulata as the target, C. semiserata, C. polyodonta and C. japonica as probe respectively. Almost one third chromosomes of hexaploid C. reticulata have strong signals at the whole genome, with diploid C. reticulata, C. pitardii and C. saluenensis as probe respectively. The results supported that diploid C. reticulata, C. pitardii and C. saluenensis are the diploid progenitors of hexaploid C. reticulata.
6, There are signals at the end of chromosomes with the diploid C. reticulata as target, C. pitardii, C. saluenensis and C. japonica as probe respectively.
7, The GISH results proved that C. reticulata hexaploid contained one set of C. reticulata diploid, one set of C. pitardii and one set of C. saulensis and part set of C. japonica and some close realtive species.|