Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation
Chen, Qi1,2; Yang, Liming1,3; Ahmad, Parvaiz4; Wan, Xiaochun2; Hu, Xiangyang1
2011-03-01
发表期刊PLANTA
ISSN0032-0935
卷号233期号:3页码:583-592
摘要Tea seed is believed to be recalcitrant based on its sensitivity to chilling or drying stress. Reactive oxygen species (ROS) and alterations in cytosolic redox status have been implicated in intolerance to desiccation by recalcitrant seed, but there is little information available regarding how ROS are regulated in seeds susceptible to drying stress. We investigated changes in protein expression and activity in tea embryo in response to desiccation using physiological and proteomic methods. Results showed that desiccation treatment dramatically induced the accumulation of H2O2 in tea embryos, accompanied by increased activities of antioxidant enzymes like ascorbate peroxidase (APX) and superoxide dismutase (SOD). Proteomic analyses also demonstrated that 23 proteins associated with defense response, metabolism and redox status were up-regulated following desiccation. Increase in antioxidants, ascorbic acid (AsA) and catalase (CAT) (H2O2 scavengers) partially assuaged desiccation damage to tea seed, resulting in improved germination rates. Higher accumulation of H2O2 aggravated desiccation damage to seeds leading to lower germination activity. We propose that desiccation causes an over-accumulation of ROS that are not efficiently scavenged by increased levels of antioxidant enzymes. High levels of ROS alter the redox status and are detrimental to seed viability. Reducing ROS to appropriate concentrations is an efficient way to reduce desiccation damage and improve germination rates of recalcitrant seeds.
关键词Camellia Desiccation Proteomic Reactive Oxygen Species Recalcitrant Seeds
资助信息CAS; National Science Foundation of China[30871704, 30971452, 30900871]
学科领域Plant Sciences
DOI10.1007/s00425-010-1322-7
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000287666300012
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3834
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Inst Tibet Plateau Res Kunming, Kunming 650204, Peoples R China
2.Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Hefei 230036, Anhui, Peoples R China
3.Huaiyin Normal Univ, Sch Life Sci, Huaiyin 223300, Peoples R China
4.Univ Kashmir, AS Coll, Dept Bot, Srinagar 190008, Jammu & Kashmir, India
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Chen, Qi,Yang, Liming,Ahmad, Parvaiz,et al. Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation[J]. PLANTA,2011,233(3):583-592.
APA Chen, Qi,Yang, Liming,Ahmad, Parvaiz,Wan, Xiaochun,&Hu, Xiangyang.(2011).Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation.PLANTA,233(3),583-592.
MLA Chen, Qi,et al."Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation".PLANTA 233.3(2011):583-592.
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