| A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae) | |
Zhou, Wei1,2; Li, Dezhu1,3 ; Wang, Hong1,3
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| 通讯作者 | zhouwei@mail.kib.ac.cn ; dzl@mail.kib.ac.cn ; wanghong@mail.kib.ac.cn |
| 2011-10-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| ISSN | 1661-6596 |
| 卷号 | 12期号:10页码:6743-6748 |
| 摘要 | Luculia gratissima (Wall.) Sweet (Rubiaceae) is a perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar. The plant is a distylous species with reciprocally placed stigmas and anthers in each floral morph. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing (FIASCO) repeats protocol, 19 primer sets were identified in two wild populations. Of these primers, 10 displayed polymorphisms and nine were monomorphic. The number of alleles per locus ranged from two to five, values for observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.289 to 0.760, with averages of 0.303 and 0.555, respectively. These microsatellite loci will facilitate further studies on breeding system, gene flow patterns, and population structure of L. gratissima and its allied species. |
| 关键词 | Luculia Gratissima Distylous Microsatellite Marker Polymorphism Population Genetics |
| 学科领域 | Chemistry, Multidisciplinary |
| DOI | 10.3390/ijms12106743 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296569600025 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/3818 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming 650201, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Wei,Li, Dezhu,Wang, Hong. A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(10):6743-6748. |
| APA | Zhou, Wei,Li, Dezhu,&Wang, Hong.(2011).A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(10),6743-6748. |
| MLA | Zhou, Wei,et al."A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.10(2011):6743-6748. |
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