A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)
Zhou, Wei1,2; Li, Dezhu1,3; Wang, Hong1,3
Corresponding Authorzhouwei@mail.kib.ac.cn ; dzl@mail.kib.ac.cn ; wanghong@mail.kib.ac.cn
2011-10-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
Volume12Issue:10Pages:6743-6748
AbstractLuculia gratissima (Wall.) Sweet (Rubiaceae) is a perennial shrub distributed in the southeast margin of the Tibetan Plateau in southwest China and adjacent region of Nepal and Myanmar. The plant is a distylous species with reciprocally placed stigmas and anthers in each floral morph. By using the Fast Isolation by Amplified Fragment Length Polymorphism (AFLP) of Sequences Containing (FIASCO) repeats protocol, 19 primer sets were identified in two wild populations. Of these primers, 10 displayed polymorphisms and nine were monomorphic. The number of alleles per locus ranged from two to five, values for observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.289 to 0.760, with averages of 0.303 and 0.555, respectively. These microsatellite loci will facilitate further studies on breeding system, gene flow patterns, and population structure of L. gratissima and its allied species.
KeywordLuculia Gratissima Distylous Microsatellite Marker Polymorphism Population Genetics
Subject AreaChemistry, Multidisciplinary
DOI10.3390/ijms12106743
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000296569600025
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/3818
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Wei,Li, Dezhu,Wang, Hong. A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(10):6743-6748.
APA Zhou, Wei,Li, Dezhu,&Wang, Hong.(2011).A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(10),6743-6748.
MLA Zhou, Wei,et al."A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.10(2011):6743-6748.
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