| Species clarification of oyster mushrooms in China and their DNA barcoding | |
Li, Jing1,2,3 ; He, Xing1,2; Liu, Xiao-Bin1,2; Yang, Zhu L.1; Zhao, Zhi-Wei3
| |
| 2017-03-01 | |
| 发表期刊 | MYCOLOGICAL PROGRESS
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| 卷号 | 16期号:3页码:191-203 |
| 摘要 | Species of the Pleurotus ostreatus complex (oyster mushrooms) are one of the main groups of cultivated edible mushrooms in China, with abundant strains and a complex genetic background. Although the cultivation of oyster mushrooms is a very important industry in China, the inconsistent nomenclature of the complex is controversial and has led to much confusion and economic loss in the mushroom industry. In this study, we performed molecular identification of the species complex based on sequences of the internal transcribed spacer region (ITS), translation elongation factor 1-alpha (TEF1), and two genes that encode subunits of RNA polymerase II (RPB1 and RPB2). A total of 284 samples with different commercial names gathered from different mushroom spawn preservation centers, companies, and field isolations were investigated via phylogenetic analyses inferred from a single locus or multi-loci. Our analyses indicated that 56% of the strains were labeled with improper scientific names and that all of the strains could be divided into seven lineages, representing one morphological species each, namely P. abieticola, P. eryngii, P. cf. floridanus, P. ostreatus, P. placentodes, P. pulmonarius, and P. tuoliensis. Reference sequences for accurate identification of the taxa were developed or suggested. Meanwhile, we evaluated the suitability of the four candidate segments as DNA barcodes. Our analyses indicated that RPB2 was the most promising candidate segment of DNA barcode for the P. ostreatus species complex, taking into consideration the polymorphisms and other aspects of the four markers. |
| 关键词 | Phylogenetic Analyses Species Recognition Strain Identification Dna Sequence Markers |
| DOI | 10.1007/s11557-016-1266-9 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394451000001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/33832 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yunnan Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming 650091, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jing,He, Xing,Liu, Xiao-Bin,et al. Species clarification of oyster mushrooms in China and their DNA barcoding[J]. MYCOLOGICAL PROGRESS,2017,16(3):191-203. |
| APA | Li, Jing,He, Xing,Liu, Xiao-Bin,Yang, Zhu L.,&Zhao, Zhi-Wei.(2017).Species clarification of oyster mushrooms in China and their DNA barcoding.MYCOLOGICAL PROGRESS,16(3),191-203. |
| MLA | Li, Jing,et al."Species clarification of oyster mushrooms in China and their DNA barcoding".MYCOLOGICAL PROGRESS 16.3(2017):191-203. |
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| art%3A10.1007%2Fs115（1561KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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