|其他题名||Studies on Asexual Reproduction of Camellia reticulata and Cytological Researches of Several Camellia Species
杜鹃红山茶（Camellia azalea）、越南抱茎山茶（Camellia amplexicaulis）和全缘叶山茶（Camellia subintegra）的体细胞中期染色体数目均为2n = 2x = 30，为二倍体；核型类型分别属于Stebbins的1B、2B和2A型。 黑牛山红花油茶（C. reticulata f. simplex）的体细胞中期染色体数目为2n = 6x = 90，属于比较进化的六倍体。|
|其他摘要||The species Camellia reticulata (Theaceae) is endemic to China. It is a world famous species with great ornamental value. Studies of asexual propagation of cutting as well as tissue culture on the species were carried out and the results were reported in this paper. In addition, cytological researches on several species of Camellia were also conducted and reported in the paper. The main progresses were summarized as follows:
1. Cutting propagation
The orthogonal table L9(34) was designed for the cutting experiment of C. reticulata ‘Juban’, including 4 factors, 3 levels and 9 treatments. The results indicated that among 4 factors, the cutting media had strongest influence on cutting propagation, possibly caused by their pH values. The best combination was achieved in 9 treatments, namely using local laterite as cutting medium, soaked in IBA500mg/L for 4 hours as well as dipped with PVP powder while cutting. The best combination calculated by orthogonal experiment was the one using local laterite as cutting medium, soaked in IBA500mg/L for 4 hours as well as dipped with rooting powder while cutting.
The experiments on rooting inhibitor were measured together with a validate experiment, then a correlation analysis was carried out. The results indicated that high contents of polyphenol and condensed tannin could be the main factor why C. reticulata was hard to be propagated by cutting. And the rooting ratio is positive correlation with the ratio of carbohydrate to condensed tannin (C/T). The higher ratio resulted easier rooting for cutting propagation. The rooting ratio was negative correlation with the contents of condensed tannin when the amount of carbohydrate was the same.
2. Tissue culture
Lower ratio of explants contaminated and good generating callus of explants were achieved when explants were successionally used Ethanol, Sodium hypochlorous (NaClO3 ) and Hydrargyrum chloride (HgCl2) as sterilized solutions. The experiments of adding hormone showed that a certain concentration of hormone combination benefited the generation of callus in C. reticulata. The reason why the buds cultivated were not able to open was that the scales wrapped the leaf buds too tightly. When using leaf bud as an explant and to avoid becoming brown, it is an effective way of peeling the scales after the callus forming at the base of buds. The reason could be possibly because the activity of Polyphenol oxidases was gradually depressed when they were removed from their mother body.
3. Cytological study
The chromosome number of Camellia azalea, C. amplexicaulis and C. subintegra were 30 in somatic cells, belonging to Stebbins’ 1B, 2B and 2A respectively. All of them are diploids. The plants of C. reticulata f. simplex in the Heiniu Mountain of Chuxiong, Yunnan were hexaploids, having 90 chromosomes in somatic cells.|
王霜. 云南山茶的无性繁殖及几种山茶属植物的细胞学研究[D]. 昆明植物研究所. 中国科学院昆明植物研究所,2008.