|其他题名||Tissue Culture and Germplasm Preservation in vitro of several Pleione species
|其他摘要||In the present thesis, the main contents focus on the following two aspects. First part, field survey and market research were performed to learn the situation of medicinal plant resources in Southeast Yunnan in China. The main medicinal plants used by local residents were listed. The degree of threaten and the conservation priority of local rare medicinal plants were ranked by using a method of synthetic assessment. According to the results, local special plant, several Pleione species were determined to be use as experimental material of tissue culture and germplasm conservation in vitro. Second, the preliminary study in tissue culture technolgy and the effective preservation method in vitro of several Pleione species were investigated. The main results of the present study are as follows.
1. Medicinal plants resources are rich in Southeast Yunnan, which is related to unique topographty and climate character in the region. The preliminary inventory of main medicinal plants used frequently by local people has been accomplished, which includes 220 medicinal plant species in 92 plant families are recorded and their scientific name, local names, living habitat, the popular uses, the part of plant used, the preparation and mode of administration are presented in the thesis.
2. The evaluation of the rare species of medicinal plants was conducted during the survey in the field and market. According to the results, we selected some rare species of medicinal plants for following study and ranked them in terms of the degree of threaten and conservation priority based on the method of synthetic assessment. We decided to use several Pleione species as experimental material of tissue culture and preservation in vitro.
3. The proper culture conditions of seed germination, protocorm multiplication, buds inducement and growth and rooting of several Pleione species were studied in the experiment, and the rapid propagation system is established to obtain large numbers of test-tube seedlings. 1) The results of the seed germination show that germination percentage is impacted highly by maturity degree of fruits. The germination percentage is 99% if the fruit has grown for 120 days. Medium of HA with 2% sugar is the best medium for seed germination. 2) The Orthogonal Design was adopted to arrange experiments of protocorm multiplication and cluster buds inducement and growth, and all the data were analyzed by SPSS. The results shows that the main effect factor of protocorm multiplication and buds inducement is the sugar concentration. To certain extent, the propagation of protocorm and cluster buds is accelerated by the high concentration of BA. The medium 1/2 MS with relative high concentrated BA and low NAA is favorable for strengthening young plants. 3) The study in producing roots of test-tube seedlings indicated that the positive effect of NAA is superior to that of IBA. High BA concentration makes against inducing roots. 4) High concentration of inorganic salt in mediums had a better effect on growth of young plants. The seedlings must be forged for 5 days in the room before they were transplanted. After being transplanted, the seedlings should be overshadowed and kept warm. Humic soil mixing with stone (1:3) was suitable for transplanting seedlings. The survival rate reached to 90% and the seedlings grew strongly.
4. Three methods, including keeping low temperature, low nutrition supply and adding growth depressor were used to preserve test-tube seedling. Living ability of material preserved were tested.|
易瑾. 独蒜兰属几种植物的组织培养和种质离体保存技术的研究[D]. 昆明植物研究所. 中国科学院昆明植物研究所,2008.