Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting

doi:10.1007/s10722-010-9571-7

	Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting
	Yang, Yang1 ; Pan, Yuezhi1 ; Gong, Xun1 ; Fan, Moutian 2
通讯作者	gongxun@mail.kib.ac.cn
	2010-12-01
发表期刊	GENETIC RESOURCES AND CROP EVOLUTION
ISSN	0925-9864
卷号	57 期号:8 页码:1239-1248
摘要	Citrus hongheensis is a critically endangered species endemic to the Honghe river region in southeastern Yunnan, China. Its genetic diversity and differentiation were investigated using Inter-Simple Sequence Repeat (ISSR) markers. One hundred primers were screened, and a total of 245 loci were amplified from seven natural populations by 13 informative and reliable primers. Of these 245 ISSR loci, 233 were polymorphic and the detected variations revealed a relatively high level of intraspecific genetic diversity. At the population level, the mean percentage of polymorphic loci (PPB) was 36.50%, while the average expected heterozygosity (He) and Shannon diversity index (Ho) were 0.1327 and 0.1972, respectively. At the species level (across all populations), PPB was 95.10%, while He and Ho were 0.3520 and 0.5195, respectively. A high Gst value (0.6247) indicated that there is significant differentiation among populations, which was confirmed by AMOVA analysis (I broken vertical bar st = 0.6420). Pairwise genetic identity (I) values among populations ranged from 0.6341 to 0.7675, with a mean of 0.7008. We propose that the high level of genetic differentiation may be the result of habitat fragmentation and limited gene flow (Nm = 0.1502). For effective in situ conservation and population restoration of C. hongheensis it will be important to maintain historical processes, including high outbreeding rates, sufficient gene flow, and large effective population sizes.
关键词	Citrus Hongheensis Conservation Differentiation Genetic Diversity Issr Fingerprinting Rutaceae
学科领域	Agronomy ; Plant Sciences
DOI	10.1007/s10722-010-9571-7
收录类别	SCI
语种	英语
WOS记录号	WOS:000284777400012
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/3180
专题	中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China 2.Yunnan Agr Univ, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Yang, Yang,Pan, Yuezhi,Gong, Xun,et al. Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting[J]. GENETIC RESOURCES AND CROP EVOLUTION,2010,57(8):1239-1248.
APA	Yang, Yang,Pan, Yuezhi,Gong, Xun,&Fan, Moutian.(2010).Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting.GENETIC RESOURCES AND CROP EVOLUTION,57(8),1239-1248.
MLA	Yang, Yang,et al."Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting".GENETIC RESOURCES AND CROP EVOLUTION 57.8(2010):1239-1248.

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