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题名: Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting
作者: Yang, Yang1; Pan, Yuezhi1; null(龚洵)1; Fan, Moutian2
刊名: GENETIC RESOURCES AND CROP EVOLUTION
关键词: Citrus hongheensis ; Conservation ; Differentiation ; Genetic diversity ; ISSR fingerprinting ; Rutaceae
英文摘要: Citrus hongheensis is a critically endangered species endemic to the Honghe river region in southeastern Yunnan, China. Its genetic diversity and differentiation were investigated using Inter-Simple Sequence Repeat (ISSR) markers. One hundred primers were screened, and a total of 245 loci were amplified from seven natural populations by 13 informative and reliable primers. Of these 245 ISSR loci, 233 were polymorphic and the detected variations revealed a relatively high level of intraspecific genetic diversity. At the population level, the mean percentage of polymorphic loci (PPB) was 36.50%, while the average expected heterozygosity (He) and Shannon diversity index (Ho) were 0.1327 and 0.1972, respectively. At the species level (across all populations), PPB was 95.10%, while He and Ho were 0.3520 and 0.5195, respectively. A high Gst value (0.6247) indicated that there is significant differentiation among populations, which was confirmed by AMOVA analysis (I broken vertical bar st = 0.6420). Pairwise genetic identity (I) values among populations ranged from 0.6341 to 0.7675, with a mean of 0.7008. We propose that the high level of genetic differentiation may be the result of habitat fragmentation and limited gene flow (Nm = 0.1502). For effective in situ conservation and population restoration of C. hongheensis it will be important to maintain historical processes, including high outbreeding rates, sufficient gene flow, and large effective population sizes.
出版日期: 2010-12-01
卷号: 57, 期号:8, 页码:1239-1248
DOI标识: 10.1007/s10722-010-9571-7
语种: 英语
收录类别: SCI
学科分类: Agronomy; Plant Sciences
ISSN号: 0925-9864
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/3180
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China
2.Yunnan Agr Univ, Kunming 650201, Peoples R China

Recommended Citation:
Yang, Y; Pan, YZ; Gong, X; Fan, MT.Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting,GENETIC RESOURCES AND CROP EVOLUTION,2010,57(8):1239-1248
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