Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region | |
Ai, Hong-Lian1,2; Wang, Hong1,3; Li, De-Zhu1; Yang, Jun-Bo1 | |
通讯作者 | wanghong@mail.kib.ac.cn |
2009-10-01 | |
发表期刊 | CONSERVATION GENETICS |
ISSN | 1566-0621 |
卷号 | 10期号:5页码:1613-1615 |
摘要 | Incarvillea mairei (H. Leveille) Grierson (Bignoniaceae) is an endemic species to the Himalaya-Hengduan Mountains region. Here, we developed 13 microsatellite markers from I. mairei using a modified biotin-streptavidin capture method. Number of alleles per locus (NA) ranged from 3 to 7 with an average of 4.615. The observed (H(O)) and expected (H(E)) heterozygosities were from 0.050 to 0.800 and from 0.249 to 0.815, respectively. Additionally, among the 13 identified microsatellite markers, 12 of them were successfully amplified in other three congeneric species, and most of them showed polymorphic. Obtained evidences suggest that these markers provide a useful tool for further study of the population genetic structure and the breeding system in this species or/and infra-generic species. |
关键词 | Incarvillea Mairei Genetic Structure Breeding System Microsatellites Himalaya-hengduan Mountains Region |
学科领域 | Biodiversity Conservation ; Genetics & Heredity |
DOI | 10.1007/s10592-009-9808-1 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000270737400075 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/3148 |
专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Nanjing Univ, Sch Life Sci, Nanjing 210093, Peoples R China |
推荐引用方式 GB/T 7714 | Ai, Hong-Lian,Wang, Hong,Li, De-Zhu,et al. Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region[J]. CONSERVATION GENETICS,2009,10(5):1613-1615. |
APA | Ai, Hong-Lian,Wang, Hong,Li, De-Zhu,&Yang, Jun-Bo.(2009).Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region.CONSERVATION GENETICS,10(5),1613-1615. |
MLA | Ai, Hong-Lian,et al."Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region".CONSERVATION GENETICS 10.5(2009):1613-1615. |
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