Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region
Ai, Hong-Lian1,2; Wang, Hong1,3; Li, De-Zhu1; Yang, Jun-Bo1
2009-10-01
发表期刊CONSERVATION GENETICS
ISSN1566-0621
卷号10期号:5页码:1613-1615
摘要Incarvillea mairei (H. Leveille) Grierson (Bignoniaceae) is an endemic species to the Himalaya-Hengduan Mountains region. Here, we developed 13 microsatellite markers from I. mairei using a modified biotin-streptavidin capture method. Number of alleles per locus (NA) ranged from 3 to 7 with an average of 4.615. The observed (H(O)) and expected (H(E)) heterozygosities were from 0.050 to 0.800 and from 0.249 to 0.815, respectively. Additionally, among the 13 identified microsatellite markers, 12 of them were successfully amplified in other three congeneric species, and most of them showed polymorphic. Obtained evidences suggest that these markers provide a useful tool for further study of the population genetic structure and the breeding system in this species or/and infra-generic species.
关键词Incarvillea Mairei Genetic Structure Breeding System Microsatellites Himalaya-hengduan Mountains Region
资助信息National Basic Research Program of China [2007CB411603]
学科领域Biodiversity Conservation ; Genetics & Heredity
DOI10.1007/s10592-009-9808-1
收录类别SCI
语种英语
WOS研究方向Biodiversity & Conservation ; Genetics & Heredity
WOS类目Biodiversity Conservation ; Genetics & Heredity
WOS记录号WOS:000270737400075
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/3148
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Nanjing Univ, Sch Life Sci, Nanjing 210093, Peoples R China
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Ai, Hong-Lian,Wang, Hong,Li, De-Zhu,et al. Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region[J]. CONSERVATION GENETICS,2009,10(5):1613-1615.
APA Ai, Hong-Lian,Wang, Hong,Li, De-Zhu,&Yang, Jun-Bo.(2009).Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region.CONSERVATION GENETICS,10(5),1613-1615.
MLA Ai, Hong-Lian,et al."Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region".CONSERVATION GENETICS 10.5(2009):1613-1615.
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