| Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum | |
Zhao, Yin-He1,2,3; Moeller, Michael4; Yang, Jun-Bo1; Liu, Ting-Song5; Zhao, Jin-Feng5; Dong, Li-Na1; Zhang, Jin-Peng6; Li, Cheng-Yun2,3; Wang, Guo-Ying6; Li, De-Zhu1
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| 通讯作者 | yhzhao808@163.com ; gywang@cau.edu.cn ; dzl@mail.kib.ac.cn |
| 2010 | |
| 发表期刊 | PLANTA
 |
| ISSN | 0032-0935 |
| 卷号 | 231期号:2页码:265-276 |
| 摘要 | Asarum caudigerum (Aristolochiaceae) is a paleoherb species that is important for research in origin and evolution of angiosperm flowers due to its basal position in the angiosperm phylogeny. In this study, a subtracted floral cDNA library from floral buds of A. caudigerum was constructed and cDNA arrays by suppression subtractive hybridization were generated. cDNAs of floral buds at different stages before flower opening and of leaves at the seedling stage were used. The macroarray analyses of expression profiles of isolated floral genes showed that 157 genes out of the 612 unique ESTs tested revealed higher transcript abundance in the floral buds and uppermost leaves. Among them, 78 genes were determined to be differentially expressed in the perianth, 62 in the stamens, and 100 genes in the carpels. Quantitative real-time PCR of selected genes validated the macroarray results. Remarkably, APETALA3 (AP3) B-class genes isolated from A. caudigerum were upregulated in the perianth, stamens and carpels, implying that the expression domain of B-class genes in this basal angiosperm was broader than those in their eudicot counterparts. |
| 关键词 | B-class Mads-box Genes Floral Gene Expression Cdna Macroarray Phylogenetic Analysis |
| 学科领域 | Plant Sciences |
| DOI | 10.1007/s00425-009-1048-6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273175900005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/3108 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China 2.Yunnan Agr Univ, Coll Agron, Kunming 650201, Peoples R China 3.Yunnan Agr Univ, Key Lab Plant Pathol Yunnan Prov, Kunming 650201, Peoples R China 4.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland 5.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China 6.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yin-He,Moeller, Michael,Yang, Jun-Bo,et al. Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum[J]. PLANTA,2010,231(2):265-276. |
| APA | Zhao, Yin-He.,Moeller, Michael.,Yang, Jun-Bo.,Liu, Ting-Song.,Zhao, Jin-Feng.,...&Li, De-Zhu.(2010).Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum.PLANTA,231(2),265-276. |
| MLA | Zhao, Yin-He,et al."Extended expression of B-class MADS-box genes in the paleoherb Asarum caudigerum".PLANTA 231.2(2010):265-276. |
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