| PanB is involved in nicotine metabolism in Pseudomonas putida | |
Wei, Hailei1,2; Lei, Liping3; Liu, Shuo4; Xia, Zhenyuan3; Liu, Xingzhong1; Liu, Peigui2
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| 通讯作者 | liuxz@im.ac.cn ; pgliu@kib.ac.cn |
| 2009-12-01 | |
| 发表期刊 | INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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| ISSN | 0964-8305 |
| 卷号 | 63期号:8页码:988-992 |
| 摘要 | A nicotine-sensitive mutant was generated from the nicotine-degrading bacterium, Pseudomonas putida strain J5, by mini-Tn5 transposon mutagenesis. This mutant was unable to grow with nicotine as the sole carbon source but could grow with glucose. Sequence analysis showed that the Tn5 transposon inserted at the site of the ketopantoate hydroxymethyltransferase gene (panB), which had 54% identity to PanB in Escherichia coli K-12. In-frame deletion of the panB gene abolished the nicotine-degrading ability of strain J5, while complementation with panB from P putida J5 and E. coli K-12 restored the degrading activity of the mutant to the wild-type level. These results suggest that ketopantoate hydroxymethyltransferase is a crucial enzyme in nicotine metabolism in P putida J5. (C) 2009 Elsevier Ltd. All rights reserved. |
| 关键词 | Biodegradation Nicotine Pseudomonas Putida Ketopantoate Hydroxymethyltransferase |
| 学科领域 | Biotechnology & Applied Microbiology ; Environmental Sciences |
| DOI | 10.1016/j.ibiod.2009.02.010 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272218800004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/2984 |
| 专题 | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Key Lab Systemat Mycol & Lichenol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China 3.Yunnan Tobacco Res Inst, Yuxi, Peoples R China 4.Beijing Jiaotong Univ, Affiliated High Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Hailei,Lei, Liping,Liu, Shuo,et al. PanB is involved in nicotine metabolism in Pseudomonas putida[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2009,63(8):988-992. |
| APA | Wei, Hailei,Lei, Liping,Liu, Shuo,Xia, Zhenyuan,Liu, Xingzhong,&Liu, Peigui.(2009).PanB is involved in nicotine metabolism in Pseudomonas putida.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,63(8),988-992. |
| MLA | Wei, Hailei,et al."PanB is involved in nicotine metabolism in Pseudomonas putida".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 63.8(2009):988-992. |
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