中国科学院昆明植物研究所机构知识库
Advanced  
KIB OpenIR  > 中国科学院东亚植物多样性与生物地理学重点实验室  > 期刊论文
题名: PanB is involved in nicotine metabolism in Pseudomonas putida
作者: Wei, Hailei1, 2; Lei, Liping3; Liu, Shuo4; Xia, Zhenyuan3; Liu, Xingzhong1; Liu, Peigui2
刊名: INTERNATIONAL BIODETERIORATION & BIODEGRADATION
关键词: Biodegradation ; Nicotine ; Pseudomonas putida ; Ketopantoate hydroxymethyltransferase
英文摘要: A nicotine-sensitive mutant was generated from the nicotine-degrading bacterium, Pseudomonas putida strain J5, by mini-Tn5 transposon mutagenesis. This mutant was unable to grow with nicotine as the sole carbon source but could grow with glucose. Sequence analysis showed that the Tn5 transposon inserted at the site of the ketopantoate hydroxymethyltransferase gene (panB), which had 54% identity to PanB in Escherichia coli K-12. In-frame deletion of the panB gene abolished the nicotine-degrading ability of strain J5, while complementation with panB from P putida J5 and E. coli K-12 restored the degrading activity of the mutant to the wild-type level. These results suggest that ketopantoate hydroxymethyltransferase is a crucial enzyme in nicotine metabolism in P putida J5. (C) 2009 Elsevier Ltd. All rights reserved.
出版日期: 2009-12-01
卷号: 63, 期号:8, 页码:988-992
DOI标识: 10.1016/j.ibiod.2009.02.010
语种: 英语
收录类别: SCI
学科分类: Biotechnology & Applied Microbiology; Environmental Sciences
ISSN号: 0964-8305
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/2984
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
201112200034.pdf(453KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Microbiol, Key Lab Systemat Mycol & Lichenol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China
3.Yunnan Tobacco Res Inst, Yuxi, Peoples R China
4.Beijing Jiaotong Univ, Affiliated High Sch, Beijing, Peoples R China

Recommended Citation:
Wei, HL; Lei, LP; Liu, S; Xia, ZY; Liu, XZ; Liu, PG.PanB is involved in nicotine metabolism in Pseudomonas putida,INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2009,63(8):988-992
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wei, Hailei]'s Articles
[Lei, Liping]'s Articles
[Liu, Shuo]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wei, Hailei]‘s Articles
[Lei, Liping]‘s Articles
[Liu, Shuo]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 201112200034.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院昆明植物研究所 - Feedback
Powered by CSpace