PanB is involved in nicotine metabolism in Pseudomonas putida
Wei, Hailei1,2; Lei, Liping3; Liu, Shuo4; Xia, Zhenyuan3; Liu, Xingzhong1; Liu, Peigui2
2009-12-01
发表期刊INTERNATIONAL BIODETERIORATION & BIODEGRADATION
ISSN0964-8305
卷号63期号:8页码:988-992
摘要A nicotine-sensitive mutant was generated from the nicotine-degrading bacterium, Pseudomonas putida strain J5, by mini-Tn5 transposon mutagenesis. This mutant was unable to grow with nicotine as the sole carbon source but could grow with glucose. Sequence analysis showed that the Tn5 transposon inserted at the site of the ketopantoate hydroxymethyltransferase gene (panB), which had 54% identity to PanB in Escherichia coli K-12. In-frame deletion of the panB gene abolished the nicotine-degrading ability of strain J5, while complementation with panB from P putida J5 and E. coli K-12 restored the degrading activity of the mutant to the wild-type level. These results suggest that ketopantoate hydroxymethyltransferase is a crucial enzyme in nicotine metabolism in P putida J5. (C) 2009 Elsevier Ltd. All rights reserved.
关键词Biodegradation Nicotine Pseudomonas Putida Ketopantoate Hydroxymethyltransferase
资助信息National Natural Science Foundation of China [30460007, 30760011]; Science and Technology Program of the Yunnan Tobacco Co. Ltd [04A17]
学科领域Biotechnology & Applied Microbiology ; Environmental Sciences
DOI10.1016/j.ibiod.2009.02.010
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology
WOS类目Biotechnology & Applied Microbiology ; Environmental Sciences
WOS记录号WOS:000272218800004
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2984
专题中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Inst Microbiol, Key Lab Systemat Mycol & Lichenol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China
3.Yunnan Tobacco Res Inst, Yuxi, Peoples R China
4.Beijing Jiaotong Univ, Affiliated High Sch, Beijing, Peoples R China
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Wei, Hailei,Lei, Liping,Liu, Shuo,et al. PanB is involved in nicotine metabolism in Pseudomonas putida[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2009,63(8):988-992.
APA Wei, Hailei,Lei, Liping,Liu, Shuo,Xia, Zhenyuan,Liu, Xingzhong,&Liu, Peigui.(2009).PanB is involved in nicotine metabolism in Pseudomonas putida.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,63(8),988-992.
MLA Wei, Hailei,et al."PanB is involved in nicotine metabolism in Pseudomonas putida".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 63.8(2009):988-992.
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