Purification and characterization of a new ribosome inactivating protein from cinchonaglycoside C-treated tobacco leaves

doi:10.1111/j.1672-9072.2007.00476.x

	Purification and characterization of a new ribosome inactivating protein from cinchonaglycoside C-treated tobacco leaves
	Li, Yanmei ; Jia, Yantao ; Zhang, Zhongkai ; Chen, Xiaoying ; He, Hongping ; Fang, Rongxiang ; Hao, Xiaojiang
通讯作者	haoxj@mail.kit.ac.cn ; fangrx@sun.im.ac.cn
	2007-09-01
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	49 期号:9 页码:1327-1333
摘要	A new ribosome-in activating protein (RIP) with a molecular weight of 31 kDa induced by Cinchonaglycoside C (1) designated CIP31, was isolated from tobacco leaves. Analysis of this protein sequence indicated that it belongs to the RIP family and it was distinct from the other plant RIPs reported previously at its N-terminal amino acid sequence. CIP31 can directly impair synthesis of coat protein (CP) of tobacco mosaic virus JMV), which resulted in inhibition of TMV long distance movement and multiplication in tobacco plants at concentrations of ng/mL. Furthermore, no toxicity was shown to the growth and fertility of the plants. CIP31 was synthesized only in the presence of Cinchonaglycoside C (1) and was independent of the salicylic acid (SA) signal pathway. We provided evidence for the SA-independent biological induction of resistance.
关键词	Antiviral Protein Cinchonaglycoside c (1) Coat Protein Medical Plant Ribosome Inactivating Protein Salicylic Acid Tobacco Mosaic Virus
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1111/j.1672-9072.2007.00476.x
收录类别	SCI
语种	英语
WOS记录号	WOS:000249268200007
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/2957
专题	植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Photochem & Plant Resources W China, Kunming 650204, Peoples R China 3.Yunnan Acad Agr Sci, Lab Plant Virol, Kunming 650223, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Yanmei,Jia, Yantao,Zhang, Zhongkai,et al. Purification and characterization of a new ribosome inactivating protein from cinchonaglycoside C-treated tobacco leaves[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2007,49(9):1327-1333.
APA	Li, Yanmei.,Jia, Yantao.,Zhang, Zhongkai.,Chen, Xiaoying.,He, Hongping.,...&Hao, Xiaojiang.(2007).Purification and characterization of a new ribosome inactivating protein from cinchonaglycoside C-treated tobacco leaves.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,49(9),1327-1333.
MLA	Li, Yanmei,et al."Purification and characterization of a new ribosome inactivating protein from cinchonaglycoside C-treated tobacco leaves".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 49.9(2007):1327-1333.

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