|其他摘要||The chemical constituents of ten macrofungi (fruiting bodies or fermentation broth) were investigated. These fungi, including Tylopilus virens, Ganoderma applanatum, Tricholomopsis rutilans, Daldinia concentrica, Cortinarius vibratilis, Albatrellus confluens, Calodon suaveolens, Agaricus silvaticus, Engleromycete goetzei, and Laccaria laccata, are all collected from Yunnan Province, China.
More than eighty compounds, including sixteen novel ones, were obtained using varied chromatography methods (CC、TLC、prep. TLC、MPLC、HPLC etc.), and the structures of the new compounds were elucidated on the basis of extensive spectroscopic analysis (1D NMR、2D NMR、MS、HR-MS、UV/Vis etc.). These new natural products involve novel doubly linked glycosides derived from pulvinic acid, highly oxygenated triterpenoid acids, unprecedented branched dolichol, benzopyran-4-one derivatives, ergosteryl esters or glycosides, etc.
A series of unique doubly linked glycosides derived from pulvinic acid, tylopilins A~D, were isolated from the fruiting bodies of T. virens. An unprecedented pentos-2-ulofuranose within these molecules is doubly linked to its aglycone through glycosidic and hemiketal bonds. This type of compounds was found for the first time from nature, and these constituents are responsible for the yellow bottom of the stipe.
Ganodolichol, the first example of branched dolichol, was found in the fungus G. applanatum. (Dolichols are polyisoprenoid lipid components of membranes of most living organisms, and known mainly for its important function in protein glycosylation.) Novel benzopyran-4-one derivatives possessing the significance of the chemotaxonomy of this species, applanatins A~B, as well as new highly oxygenated lanostane-type triterpenes, ganoderic acid AP2~AP3, were also isolated from this fungus.|