| Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates | |
Wang, Jiancai1,2; Xu, Ronghua3; Wang, Ruling1; Haque, Mohammad Enamul4; Liu, Aizhong1,4
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| 2016 | |
| 发表期刊 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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| 卷号 | 80期号:6页码:1214-1222 |
| 摘要 | The conversion of acetyl-CoA to malonyl-CoA by acetyl-CoA carboxylase (ACC) is the rate-limiting step in fatty acid biosynthesis. In this study, a gene coding for ACC was isolated and characterized from an oleaginous yeast, Lipomyces starkeyi. Real-time quantitative PCR (qPCR) analysis of L. starkeyi acetyl-CoA carboxylase gene (LsACC1) showed that the expression levels were upregulated with the fast accumulation of lipids. The LsACC1 was co-overexpressed with the glycerol 3-phosphate dehydrogenase gene (GPD1), which regulates lipids biosynthesis by supplying another substrates glycerol 3-phosphate for storage lipid assembly, in the non-oleaginous yeast Saccharomyces cerevisiae. Further, the S. cerevisiae acetyl-CoA carboxylase (ScACC1) was transferred with GPD1 and its function was analyzed in comparison with LsACC1. The results showed that overexpressed LsACC1 and GPD1 resulted in a 63% increase in S. cerevisiae. This study gives new data in understanding of the molecular mechanisms underlying the regulation of fatty acids and lipid biosynthesis in yeasts. |
| 关键词 | Lipomyces Starkeyi Acetyl-coa Carboxylase Glycerol 3-phosphate Dehydrogenase Fatty Acid Biosynthesis Saccharomyces Cerevisiae |
| DOI | 10.1080/09168451.2015.1136883 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000379185400027 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/28469 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei, Peoples R China 3.Anhui Sci & Technol Univ, Coll Life Sci, Fengyang, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jiancai,Xu, Ronghua,Wang, Ruling,et al. Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2016,80(6):1214-1222. |
| APA | Wang, Jiancai,Xu, Ronghua,Wang, Ruling,Haque, Mohammad Enamul,&Liu, Aizhong.(2016).Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,80(6),1214-1222. |
| MLA | Wang, Jiancai,et al."Overexpression of ACC gene from oleaginous yeast Lipomyces starkeyi enhanced the lipid accumulation in Saccharomyces cerevisiae with increased levels of glycerol 3-phosphate substrates".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 80.6(2016):1214-1222. |
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| 451.2015.1136883.pdf（990KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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