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题名: Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii
作者: Xue, Chun-Ying; null(李德铢); Lu, Jin-Mei; Yang, Jun-Bo; Liu, Jian-Quan
刊名: PLANTA MEDICA
关键词: Tibetan folk medicine ; Swertia mussotii ; Gentianaceae ; DNA sequences ; allele-specific diagnostic PCR ; authentication
英文摘要: Swertia mussotii is an important species in Tibetan folk medicine. However, it is quite expensive and frequently adulterated, so reliable methods for authentication of putative specimens and preparations of the species are needed to protect consumers and to support conservation measures. We show here that the chloroplast (cp) DNA rpl16 intron has limited utility for differentiating S. mussotii from closely related species, since the cpDNA rpl16 sequences are identical in S. mussotii and two other species of Swertia. However, the rDNA internal transcribed spacer (ITS) sequences differ significantly between S. mussotii and all of 13 tested potential adulterants. Thus, the ITS region provides a robust molecular marker for differentiating the medicinal S. mussotii from related adulterants. Therefore, a pair of allele-specific diagnostic primers based on the divergent ITS region was designed to distinguish S. mussotii from the other species. Authentication by allele-specific diagnostic PCR using these primers is convenient, effective and both simpler and less time-consuming than sequencing the ITS region.
出版日期: 2006-10-01
卷号: 72, 期号:13, 页码:1223-1226
DOI标识: 10.1055/s-2006-951695
语种: 英语
收录类别: SCI
学科分类: Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy
ISSN号: 0032-0943
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.kib.ac.cn/handle/151853/2667
Appears in Collections:中国科学院东亚植物多样性与生物地理学重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Lab Plant Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China

Recommended Citation:
Xue, CY; Li, DZ; Lu, JM; Yang, JB; Liu, JQ.Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii,PLANTA MEDICA,2006,72(13):1223-1226
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