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Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity
Wang, Lina1,2; Yang, Liming3; Yang, Fengjuan1,2; Li, Xiaogang1,2; Song, Yunpeng1,2; Wang, Xiufeng1,2; Hu, Xiangyang4
Corresponding Authorxfwang@sdau.edu.cn ; huxiangyang@mail.kib.ac.cn
2010
Source PublicationJOURNAL OF PLANT PHYSIOLOGY
ISSN0176-1617
Volume167Issue:15Pages:1298-1306
AbstractBoth H2O2 and NO are involved in multiple physiological responses in plants. Metallothionein (MT) can bind heavy metal ions and reduce metal toxicity. Copper toxicity has become a major problem with increasing agricultural and environmental pollution. Here, we investigated the possible roles of ROS, NO and metallothionein in tomato plant responses to copper toxicity. We found that Cu2+ stress caused the rapid release of H2O2 and chlorotic leaves, and it stunted root growth and development. Cu treatment also caused an increase in NOS enzyme activity and NO release in roots and leaves. Application of the NO donor SNP efficiently alleviated the copper toxicity effect, as shown by increases in chlorophyll content and the biomass of fresh/dry leaves. SNP treatment also induced the transcription and increased activities of anti-oxidant enzymes, including catalase, peroxidase, superoxide dismutase and ascorbate peroxidase, and led to reduced H2O2 accumulation in the leaves. Special inhibitors or scavengers of NO synthesis diminished the ameliorating effect of NO on copper toxicity. NO application induced MT transcription and accumulation in leaves. Furthermore, the antisense-MT transgenic tomato was more sensitive to copper stress, and this effect could not be efficiently reversed by NO treatment. From these data, we propose that NO induces tomato tolerance to copper toxicity through antioxidant enzyme activity and metallothionein accumulation, and that metallothionein acts downstream of NO signaling. (C) 2010 Elsevier GmbH. All rights reserved.; Both H2O2 and NO are involved in multiple physiological responses in plants. Metallothionein (MT) can bind heavy metal ions and reduce metal toxicity. Copper toxicity has become a major problem with increasing agricultural and environmental pollution. Here, we investigated the possible roles of ROS, NO and metallothionein in tomato plant responses to copper toxicity. We found that Cu2+ stress caused the rapid release of H2O2 and chlorotic leaves, and it stunted root growth and development. Cu treatment also caused an increase in NOS enzyme activity and NO release in roots and leaves. Application of the NO donor SNP efficiently alleviated the copper toxicity effect, as shown by increases in chlorophyll content and the biomass of fresh/dry leaves. SNP treatment also induced the transcription and increased activities of anti-oxidant enzymes, including catalase, peroxidase, superoxide dismutase and ascorbate peroxidase, and led to reduced H2O2 accumulation in the leaves. Special inhibitors or scavengers of NO synthesis diminished the ameliorating effect of NO on copper toxicity. NO application induced MT transcription and accumulation in leaves. Furthermore, the antisense-MT transgenic tomato was more sensitive to copper stress, and this effect could not be efficiently reversed by NO treatment. From these data, we propose that NO induces tomato tolerance to copper toxicity through antioxidant enzyme activity and metallothionein accumulation, and that metallothionein acts downstream of NO signaling. (C) 2010 Elsevier GmbH. All rights reserved.
KeywordCopper Hydrogen Peroxide Metallothionein Nitric Oxide Tomato
Subject AreaPlant Sciences
DOI10.1016/j.jplph.2010.04.007
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000282911300011
Citation statistics
Cited Times:44[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/2653
Collection中国科学院青藏高原研究所昆明部
Affiliation1.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China
2.State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
3.Huaiyin Normal Univ, Sch Life Sci, Huaian 223300, Peoples R China
4.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Wang, Lina,Yang, Liming,Yang, Fengjuan,et al. Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity[J]. JOURNAL OF PLANT PHYSIOLOGY,2010,167(15):1298-1306.
APA Wang, Lina.,Yang, Liming.,Yang, Fengjuan.,Li, Xiaogang.,Song, Yunpeng.,...&Hu, Xiangyang.(2010).Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity.JOURNAL OF PLANT PHYSIOLOGY,167(15),1298-1306.
MLA Wang, Lina,et al."Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity".JOURNAL OF PLANT PHYSIOLOGY 167.15(2010):1298-1306.
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