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Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity
Wang, Lina1,2; Yang, Liming3; Yang, Fengjuan1,2; Li, Xiaogang1,2; Song, Yunpeng1,2; Wang, Xiufeng1,2; Hu, Xiangyang4
2010
发表期刊JOURNAL OF PLANT PHYSIOLOGY
ISSN0176-1617
卷号167期号:15页码:1298-1306
摘要Both H2O2 and NO are involved in multiple physiological responses in plants. Metallothionein (MT) can bind heavy metal ions and reduce metal toxicity. Copper toxicity has become a major problem with increasing agricultural and environmental pollution. Here, we investigated the possible roles of ROS, NO and metallothionein in tomato plant responses to copper toxicity. We found that Cu2+ stress caused the rapid release of H2O2 and chlorotic leaves, and it stunted root growth and development. Cu treatment also caused an increase in NOS enzyme activity and NO release in roots and leaves. Application of the NO donor SNP efficiently alleviated the copper toxicity effect, as shown by increases in chlorophyll content and the biomass of fresh/dry leaves. SNP treatment also induced the transcription and increased activities of anti-oxidant enzymes, including catalase, peroxidase, superoxide dismutase and ascorbate peroxidase, and led to reduced H2O2 accumulation in the leaves. Special inhibitors or scavengers of NO synthesis diminished the ameliorating effect of NO on copper toxicity. NO application induced MT transcription and accumulation in leaves. Furthermore, the antisense-MT transgenic tomato was more sensitive to copper stress, and this effect could not be efficiently reversed by NO treatment. From these data, we propose that NO induces tomato tolerance to copper toxicity through antioxidant enzyme activity and metallothionein accumulation, and that metallothionein acts downstream of NO signaling. (C) 2010 Elsevier GmbH. All rights reserved.; Both H2O2 and NO are involved in multiple physiological responses in plants. Metallothionein (MT) can bind heavy metal ions and reduce metal toxicity. Copper toxicity has become a major problem with increasing agricultural and environmental pollution. Here, we investigated the possible roles of ROS, NO and metallothionein in tomato plant responses to copper toxicity. We found that Cu2+ stress caused the rapid release of H2O2 and chlorotic leaves, and it stunted root growth and development. Cu treatment also caused an increase in NOS enzyme activity and NO release in roots and leaves. Application of the NO donor SNP efficiently alleviated the copper toxicity effect, as shown by increases in chlorophyll content and the biomass of fresh/dry leaves. SNP treatment also induced the transcription and increased activities of anti-oxidant enzymes, including catalase, peroxidase, superoxide dismutase and ascorbate peroxidase, and led to reduced H2O2 accumulation in the leaves. Special inhibitors or scavengers of NO synthesis diminished the ameliorating effect of NO on copper toxicity. NO application induced MT transcription and accumulation in leaves. Furthermore, the antisense-MT transgenic tomato was more sensitive to copper stress, and this effect could not be efficiently reversed by NO treatment. From these data, we propose that NO induces tomato tolerance to copper toxicity through antioxidant enzyme activity and metallothionein accumulation, and that metallothionein acts downstream of NO signaling. (C) 2010 Elsevier GmbH. All rights reserved.
关键词Copper Hydrogen Peroxide Metallothionein Nitric Oxide Tomato
资助信息National Science Foundation of China [30871704, 30971452, 30900871, 30871705, 30900983]; CAS ; Natural Science Foundation of Jiangshu Higher Education Institutions in China [09KJB210001]
学科领域Plant Sciences
DOI10.1016/j.jplph.2010.04.007
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000282911300011
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2653
专题中国科学院青藏高原研究所昆明部
作者单位1.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China
2.State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
3.Huaiyin Normal Univ, Sch Life Sci, Huaian 223300, Peoples R China
4.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
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Wang, Lina,Yang, Liming,Yang, Fengjuan,et al. Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity[J]. JOURNAL OF PLANT PHYSIOLOGY,2010,167(15):1298-1306.
APA Wang, Lina.,Yang, Liming.,Yang, Fengjuan.,Li, Xiaogang.,Song, Yunpeng.,...&Hu, Xiangyang.(2010).Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity.JOURNAL OF PLANT PHYSIOLOGY,167(15),1298-1306.
MLA Wang, Lina,et al."Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity".JOURNAL OF PLANT PHYSIOLOGY 167.15(2010):1298-1306.
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