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Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana
Hu, Xiangyang1; Neill, Stevn J.2; Yang, Yongping1; Cai, Weiming3
Corresponding Authorhuxiangyang@mail.kib.ac.cn ; wmcai@sibs.ac.cn
2009-05-01
Source PublicationPLANTA
ISSN0032-0935
Volume229Issue:6Pages:1201-1208
AbstractTransgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.; Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.
KeywordArabidopsis Aequorin Cytosolic Calcium Ions Hydrogen Peroxide Octadecanoid Pathway
Subject AreaPlant Sciences
DOI10.1007/s00425-009-0909-3
Indexed BySCI
Language英语
WOS IDWOS:000265540100004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/2651
Collection中国科学院青藏高原研究所昆明部
Affiliation1.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Univ W England, Ctr Res Plant Sci, Bristol BS16 1QY, Avon, England
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 2000032, Peoples R China
Recommended Citation
GB/T 7714
Hu, Xiangyang,Neill, Stevn J.,Yang, Yongping,et al. Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana[J]. PLANTA,2009,229(6):1201-1208.
APA Hu, Xiangyang,Neill, Stevn J.,Yang, Yongping,&Cai, Weiming.(2009).Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana.PLANTA,229(6),1201-1208.
MLA Hu, Xiangyang,et al."Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana".PLANTA 229.6(2009):1201-1208.
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