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Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana
Hu, Xiangyang1; Neill, Stevn J.2; Yang, Yongping1; Cai, Weiming3
2009-05-01
发表期刊PLANTA
ISSN0032-0935
卷号229期号:6页码:1201-1208
摘要Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.; Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.
关键词Arabidopsis Aequorin Cytosolic Calcium Ions Hydrogen Peroxide Octadecanoid Pathway
资助信息National Natural Science Foundation in China [30570157]; National Natural Science Foundation [30871704]
收录类别SCI
语种英语
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/2651
专题中国科学院青藏高原研究所昆明部
作者单位1.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Univ W England, Ctr Res Plant Sci, Bristol BS16 1QY, Avon, England
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 2000032, Peoples R China
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GB/T 7714
Hu, Xiangyang,Neill, Stevn J.,Yang, Yongping,et al. Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana[J]. PLANTA,2009,229(6):1201-1208.
APA Hu, Xiangyang,Neill, Stevn J.,Yang, Yongping,&Cai, Weiming.(2009).Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana.PLANTA,229(6),1201-1208.
MLA Hu, Xiangyang,et al."Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana".PLANTA 229.6(2009):1201-1208.
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