| Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale | |
He, Tao-Bin1,2,3; Huang, Yan-Ping1,3; Yang, Liu2; Liu, Ti-Ti1,3; Gong, Wan-Ying1,3; Wang, Xuan-Jun1,3; Sheng, Jun1,3; Hu, Jiang-Miao2
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| 2016-02-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 卷号 | 83页码:34-41 |
| 摘要 | A neutral heteropolysaccharide (DOP-1-1) consisted by mannose and glucose (5.9:1) with an average molecular weight at about 1.78 x 10(5) Da was purified from Dendrobium officinale. Based on Fourier transform infrared spectrum (FT-IR) and nuclear magnetic resonance (NMR) spectra, it suggested that partial structure of DOP-1-1 is an O-acetylated glucomannan with beta-D configuration in pyranose sugar forms. The immunomodulatory activity of DOP-1-1 was evaluated by secretion level of cytokine (interleukin (IL)-1 beta and IL-10) and tumor necrosis factor (TNF)-alpha; in vitro. Our results suggested that DOP-1-1 could stimulate cytokine production (TNF-alpha, IL-1 beta) in cells. These findings demonstrated that the purified polysaccharide from D. officinale presented significant immune-modulating activities. Furthermore, by Western-blot we can found that the signaling pathways of DOP-1-1 induced immune activities involving ERK1/2 and NF-kappa B. As to antioxidant activity, DOP-1-1 hadn't showed remarkable scavenging capacity of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in contrast with other studies of polysaccharides from D. officinale. (C) 2015 Elsevier B.V. All rights reserved. |
| 关键词 | Dendrobium Officinale Polysaccharide Immunomodulation |
| DOI | 10.1016/j.ijbiomac.2015.11.038 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000369200500005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/25947 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Yunna Agr Univ, Coll Food Sci & Technol, Kunming 650201, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China 3.Yunnan Agr Univ, Minist Educ, Key Lab Pu Er Tea Sci, Kunming 650201, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Tao-Bin,Huang, Yan-Ping,Yang, Liu,et al. Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,83:34-41. |
| APA | He, Tao-Bin.,Huang, Yan-Ping.,Yang, Liu.,Liu, Ti-Ti.,Gong, Wan-Ying.,...&Hu, Jiang-Miao.(2016).Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,83,34-41. |
| MLA | He, Tao-Bin,et al."Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 83(2016):34-41. |
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