Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale
He, Tao-Bin1,2,3; Huang, Yan-Ping1,3; Yang, Liu2; Liu, Ti-Ti1,3; Gong, Wan-Ying1,3; Wang, Xuan-Jun1,3; Sheng, Jun1,3; Hu, Jiang-Miao2
2016-02-01
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷号83页码:34-41
摘要A neutral heteropolysaccharide (DOP-1-1) consisted by mannose and glucose (5.9:1) with an average molecular weight at about 1.78 x 10(5) Da was purified from Dendrobium officinale. Based on Fourier transform infrared spectrum (FT-IR) and nuclear magnetic resonance (NMR) spectra, it suggested that partial structure of DOP-1-1 is an O-acetylated glucomannan with beta-D configuration in pyranose sugar forms. The immunomodulatory activity of DOP-1-1 was evaluated by secretion level of cytokine (interleukin (IL)-1 beta and IL-10) and tumor necrosis factor (TNF)-alpha; in vitro. Our results suggested that DOP-1-1 could stimulate cytokine production (TNF-alpha, IL-1 beta) in cells. These findings demonstrated that the purified polysaccharide from D. officinale presented significant immune-modulating activities. Furthermore, by Western-blot we can found that the signaling pathways of DOP-1-1 induced immune activities involving ERK1/2 and NF-kappa B. As to antioxidant activity, DOP-1-1 hadn't showed remarkable scavenging capacity of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in contrast with other studies of polysaccharides from D. officinale. (C) 2015 Elsevier B.V. All rights reserved.
关键词Dendrobium Officinale Polysaccharide Immunomodulation
资助信息Natural Science Foundation of Yunnan Province, China(2012FB151; Foundation of Scientific and Technical Innovation of Yunnan Province, China(2009AB021; 2015FB168); 2012CG001; 2013IB021)
DOI10.1016/j.ijbiomac.2015.11.038
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号WOS:000369200500005
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/25947
专题植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Yunna Agr Univ, Coll Food Sci & Technol, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
3.Yunnan Agr Univ, Minist Educ, Key Lab Pu Er Tea Sci, Kunming 650201, Peoples R China
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He, Tao-Bin,Huang, Yan-Ping,Yang, Liu,et al. Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,83:34-41.
APA He, Tao-Bin.,Huang, Yan-Ping.,Yang, Liu.,Liu, Ti-Ti.,Gong, Wan-Ying.,...&Hu, Jiang-Miao.(2016).Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,83,34-41.
MLA He, Tao-Bin,et al."Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 83(2016):34-41.
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