Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system

doi:10.1016/j.jip.2015.11.010

	Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system
	Khan, Sehroon; Nadir, Sadia; Guo Lihua; Xu, Jianchu; Holmes, Keith A.; Qiu Dewen
	2016
发表期刊	JOURNAL OF INVERTEBRATE PATHOLOGY
卷号	133 页码:87-94
摘要	An insect-toxic protein, Bb70p, was purified from Beauveria bassiana 70 using ammonium sulfate precipitation, ion exchange chromatography, and gel filtration. Bb70p has a high affinity for anion exchangers and 2D electrophoresis results revealed a single spot with a molecular weight of 35.5 kDa and an iso-electric point of similar to 4.5. Bb70p remains active from 4 to 60 degrees C, within a pH range of 4-10, but is more active in slightly acidic pH. A pure protein, Bb70p does not have any carbohydrate side chains. The protein caused high mortality by intra-haemocelic injection into Galleria mellonella with LD50 of 334.41 mu g/g body weight and activates the phenol oxidase cascade. With a partial amino acid sequence comparison using the NCBI database, we showed no homology to known toxin proteins of entomopathogenic fungi. Thus, Bb70p appears to be an insect toxin protein, demonstrating novelty. Identification of this insect-toxic protein presents potential to enhance the virulence of B. bassiana through genetic manipulation. (C) 2015 Elsevier Inc. All rights reserved.
关键词	Insect-toxic Protein Bb70p Entomopathogenic Fungi Beauveria Bassiana Galleria Mellonella Filtrate
DOI	10.1016/j.jip.2015.11.010
收录类别	SCI
语种	英语
WOS记录号	WOS:000368317200013
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/25448
专题	资源植物与生物技术所级重点实验室
推荐引用方式 GB/T 7714	Khan, Sehroon,Nadir, Sadia,Guo Lihua,et al. Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2016,133:87-94.
APA	Khan, Sehroon,Nadir, Sadia,Guo Lihua,Xu, Jianchu,Holmes, Keith A.,&Qiu Dewen.(2016).Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system.JOURNAL OF INVERTEBRATE PATHOLOGY,133,87-94.
MLA	Khan, Sehroon,et al."Identification and characterization of an insect toxin protein, Bb70p, from the entomopathogenic fungus, Beauveria bassiana, using Galleria mellonella as a model system".JOURNAL OF INVERTEBRATE PATHOLOGY 133(2016):87-94.

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