Isolation and Functional Analysis of SpWOX13 from Stipa purpurea

doi:10.1007/s11105-014-0848-4

	Isolation and Functional Analysis of SpWOX13 from Stipa purpurea
	Sun,Xudong; Xiang,Nan; Wang,Chongde; Yang,Shihai; Li,Xiong; Yang,Yunqiang; Yang,Yongping
	2015-10-01
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
卷号	33 期号:5 页码:1441-1450
摘要	Members of the WUSCHEL-related homeobox (WOX) gene family encode plant-specific transcription factors that play key roles in controlling gene expression at all stages of plant development. In the present study, we isolated and characterized SpWOX13 (GenBank accession no. KJ713172), a member of the WOX gene family, from Stipa purpurea. Sequence and phylogenetic analyses showed that SpWOX13 belongs to an ancient clade of WOX genes. A subcellular localization analysis indicated that SpWOX13 localizes in the nucleus. The transcript levels of SpWOX13 were higher in young leaves and the vegetative apex than in other tissues of S. purpurea. Ectopic expression of SpWOX13 in Arabidopsis resulted in late flowering plants as these showed more rosette leaves. Histological analyses of the transgenic plants showed aberrant arrangements in the tunica cell layers of the shoot apical meristem. Scanning electron microscopy analyses revealed that the leaf pavement cells were larger in SpWOX13-overexpressing transgenic plants than in wild type. Our results suggest that SpWOX13 is involved in delaying and/or inhibiting cell differentiation.
关键词	Spwox13 Transcription Factor Cell Differentiation Stipa Purpurea Arabidopsis
DOI	10.1007/s11105-014-0848-4
收录类别	SCI
语种	英语
WOS记录号	WOS:000360392800022
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/25112
专题	中国科学院东亚植物多样性与生物地理学重点实验室
推荐引用方式 GB/T 7714	Sun,Xudong,Xiang,Nan,Wang,Chongde,et al. Isolation and Functional Analysis of SpWOX13 from Stipa purpurea[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015,33(5):1441-1450.
APA	Sun,Xudong.,Xiang,Nan.,Wang,Chongde.,Yang,Shihai.,Li,Xiong.,...&Yang,Yongping.(2015).Isolation and Functional Analysis of SpWOX13 from Stipa purpurea.PLANT MOLECULAR BIOLOGY REPORTER,33(5),1441-1450.
MLA	Sun,Xudong,et al."Isolation and Functional Analysis of SpWOX13 from Stipa purpurea".PLANT MOLECULAR BIOLOGY REPORTER 33.5(2015):1441-1450.

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